Linked Life Data

11901201

Source:http://linkedlifedata.com/resource/pubmed/id/11901201

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2002-3-19
pubmed:abstractText
The rae28 gene (rae28), also designated as mph1, is a mammalian ortholog of the Drosophila polyhomeotic gene, a member of Polycomb group genes (PcG). rae28 constitutes PcG complex 1 for maintaining transcriptional states which have been once initiated, presumably through modulation of the chromatin structure. Hematopoietic activity was impaired in the fetal liver of rae28-deficient animals (rae28-/-), as demonstrated by progressive reduction of hematopoietic progenitors of multilineages and poor expansion of colony forming units in spleen (CFU-S(12)) during embryonic development. An in vitro long-term culture-initiating cell assay suggested a reduction in hematopoietic stem cells (HSCs), which was confirmed in vivo by reconstitution experiments in lethally irradiated congenic recipient mice. The competitive repopulating units (CRUs) reflect HSCs supporting multilineage blood-cell production. CRUs were generated, whereas the number of CRUs was reduced by a factor of 20 in the rae28-/- fetal liver. We also performed serial transplantation experiments to semiquantitatively measure self-renewal activity of CRUs in vivo. Self-renewal activity of CRUs was 15-fold decreased in rae28-/-. Thus the compromised HSCs were presumed to reduce hematopoietic activity in the rae28-/- fetal liver. This is the first report to suggest that rae28 has a crucial role in sustaining the activity of HSCs to maintain hematopoiesis.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-10412979, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-10541554, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-10541555, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-10581039, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-10653359, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-10710306, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-10902910, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-10905048, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-11039664, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-11044623, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-11172024, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-11250141, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-11493924, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-11493925, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-11520462, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-11581156, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-13776896, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-2247442, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-2333304, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-2714405, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-5665168, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-7527557, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-7622039, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-7662368, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-7926765, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-8070621, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-8625838, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-8689561, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-8874181, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-8972230, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9009205, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9043087, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9052833, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9058712, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9121482, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9175875, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9252126, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9285685, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9309179, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9448308, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9462516, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9584197, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9584199, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9620849, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9641679, http://linkedlifedata.com/resource/pubmed/commentcorrection/11901201-9923679
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
195
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
759-70
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Polycomb group gene rae28 is required for sustaining activity of hematopoietic stem cells.
pubmed:affiliation
Department of Developmental Biology and Medicine, Osaka Medical Center for Cancer and Cardiovascular Diseases, 3-3 Nakamichi-1, Higashinari, Osaka 537-8511, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't