| pubmed-article:11897838 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11897838 | lifeskim:mentions | umls-concept:C0032824 | lld:lifeskim |
| pubmed-article:11897838 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:11897838 | lifeskim:mentions | umls-concept:C1521970 | lld:lifeskim |
| pubmed-article:11897838 | lifeskim:mentions | umls-concept:C0205419 | lld:lifeskim |
| pubmed-article:11897838 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:11897838 | lifeskim:mentions | umls-concept:C1416592 | lld:lifeskim |
| pubmed-article:11897838 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:11897838 | lifeskim:mentions | umls-concept:C0449774 | lld:lifeskim |
| pubmed-article:11897838 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:11897838 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:11897838 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:11897838 | pubmed:issue | Pt 3 | lld:pubmed |
| pubmed-article:11897838 | pubmed:dateCreated | 2002-3-18 | lld:pubmed |
| pubmed-article:11897838 | pubmed:abstractText | Two novel alternatively spliced isoforms of the human two-pore-domain potassium channel TREK-2 were isolated from cDNA libraries of human kidney and fetal brain. The cDNAs of 2438 base pairs (bp) (TREK-2b) and 2559 bp (TREK-2c) encode proteins of 508 amino acids each. RT-PCR showed that TREK-2b is strongly expressed in kidney (primarily in the proximal tubule) and pancreas, whereas TREK-2c is abundantly expressed in brain. In situ hybridization revealed a very distinct expression pattern of TREK-2c in rat brain which partially overlapped with that of TREK-1. Expression of TREK-2b and TREK-2c in human embryonic kidney (HEK) 293 cells showed that their single-channel characteristics were similar. The slope conductance at negative potentials was 163 +/- 5 pS for TREK-2b and 179 +/- 17 pS for TREK-2c. The mean open and closed times of TREK-2b at -84 mV were 133 +/- 16 and 109 +/- 11 micros, respectively. Application of forskolin decreased the whole-cell current carried by TREK-2b and TREK-2c. The sensitivity to forskolin was abolished by mutating a protein kinase A phosphorylation site at position 364 of TREK-2c (construct S364A). Activation of protein kinase C (PKC) by application of phorbol-12-myristate-13-acetate (PMA) also reduced whole-cell current. However, removal of the putative TREK-2b-specific PKC phosphorylation site (construct T7A) did not affect inhibition by PMA. Our results suggest that alternative splicing of TREK-2 contributes to the diversity of two-pore-domain K+ channels. | lld:pubmed |
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| pubmed-article:11897838 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:11897838 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11897838 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11897838 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11897838 | pubmed:issn | 0022-3751 | lld:pubmed |
| pubmed-article:11897838 | pubmed:author | pubmed-author:DautJürgenJ | lld:pubmed |
| pubmed-article:11897838 | pubmed:author | pubmed-author:DerstChristia... | lld:pubmed |
| pubmed-article:11897838 | pubmed:author | pubmed-author:SteinleinOrtr... | lld:pubmed |
| pubmed-article:11897838 | pubmed:author | pubmed-author:HirschJochen... | lld:pubmed |
| pubmed-article:11897838 | pubmed:author | pubmed-author:GuWenliW | lld:pubmed |
| pubmed-article:11897838 | pubmed:author | pubmed-author:SchlichthörlG... | lld:pubmed |
| pubmed-article:11897838 | pubmed:author | pubmed-author:EngelsHartmut... | lld:pubmed |
| pubmed-article:11897838 | pubmed:author | pubmed-author:KarschinChris... | lld:pubmed |
| pubmed-article:11897838 | pubmed:author | pubmed-author:KarschinAndre... | lld:pubmed |
| pubmed-article:11897838 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11897838 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:11897838 | pubmed:volume | 539 | lld:pubmed |
| pubmed-article:11897838 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11897838 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11897838 | pubmed:pagination | 657-68 | lld:pubmed |
| pubmed-article:11897838 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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