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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0032824,
umls-concept:C0185117,
umls-concept:C0205245,
umls-concept:C0205314,
umls-concept:C0205419,
umls-concept:C0449774,
umls-concept:C0679622,
umls-concept:C1416592,
umls-concept:C1521970,
umls-concept:C2911684
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pubmed:issue |
Pt 3
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pubmed:dateCreated |
2002-3-18
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pubmed:abstractText |
Two novel alternatively spliced isoforms of the human two-pore-domain potassium channel TREK-2 were isolated from cDNA libraries of human kidney and fetal brain. The cDNAs of 2438 base pairs (bp) (TREK-2b) and 2559 bp (TREK-2c) encode proteins of 508 amino acids each. RT-PCR showed that TREK-2b is strongly expressed in kidney (primarily in the proximal tubule) and pancreas, whereas TREK-2c is abundantly expressed in brain. In situ hybridization revealed a very distinct expression pattern of TREK-2c in rat brain which partially overlapped with that of TREK-1. Expression of TREK-2b and TREK-2c in human embryonic kidney (HEK) 293 cells showed that their single-channel characteristics were similar. The slope conductance at negative potentials was 163 +/- 5 pS for TREK-2b and 179 +/- 17 pS for TREK-2c. The mean open and closed times of TREK-2b at -84 mV were 133 +/- 16 and 109 +/- 11 micros, respectively. Application of forskolin decreased the whole-cell current carried by TREK-2b and TREK-2c. The sensitivity to forskolin was abolished by mutating a protein kinase A phosphorylation site at position 364 of TREK-2c (construct S364A). Activation of protein kinase C (PKC) by application of phorbol-12-myristate-13-acetate (PMA) also reduced whole-cell current. However, removal of the putative TREK-2b-specific PKC phosphorylation site (construct T7A) did not affect inhibition by PMA. Our results suggest that alternative splicing of TREK-2 contributes to the diversity of two-pore-domain K+ channels.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-10075682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-10206991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-10321245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-10480871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-10719894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-10725353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-10734076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-10744694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-10747866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-10747911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-10767409,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-11409881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-11567039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-11749039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-11897836,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-8938713,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-9312005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-9362342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-9506712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-9628867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-9687497,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11897838-9880510
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Recombinant,
http://linkedlifedata.com/resource/pubmed/chemical/KCNK10 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Kcnk10 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, Tandem Pore...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/potassium channel protein TREK-1
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
539
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
657-68
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11897838-Humans,
pubmed-meshheading:11897838-Animals,
pubmed-meshheading:11897838-Electrophysiology,
pubmed-meshheading:11897838-Brain,
pubmed-meshheading:11897838-Rats,
pubmed-meshheading:11897838-Genetic Variation,
pubmed-meshheading:11897838-Base Sequence,
pubmed-meshheading:11897838-RNA, Messenger,
pubmed-meshheading:11897838-Tissue Distribution,
pubmed-meshheading:11897838-Cell Line,
pubmed-meshheading:11897838-Molecular Sequence Data
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