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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2002-3-18
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pubmed:abstractText |
We have compared early signaling events at various stages of B cell differentiation using established mouse cell lines. Clustering of pre-B cell antigen receptor (BCR) or BCR induced the tyrosine phosphorylation of various proteins in all cells, although the phosphorylation pattern differed. In spite of the pre-BCR-induced tyrosine phosphorylation, we could not detect an intracellular Ca(2+) signal in pre-B cells. However, co-clustering of the pre-BCR with CD19 did induce Ca(2+) mobilization. In contrast to the immature and mature B cells, where the B cell linker protein (BLNK) went through inducible tyrosine phosphorylation upon BCR clustering, we observed a constitutive tyrosine phosphorylation of BLNK in pre-B cell lines. Both BLNK and phospholipase C (PLC)gamma were raft associated in unstimulated pre-B cells, and this could not be enhanced by pre-BCR engagement, suggesting a ligand-independent PLC gamma-mediated signaling. Further results indicate that the cell lines representing the immature stage are more sensitive to BCR-, CD19- and type II receptors binding the Fc part of IgG (Fc gamma RIIb)-mediated signals than mature B cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD19,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/B cell linker protein,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fc gamma receptor IIB,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, B-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgG,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0898-6568
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
563-72
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11897497-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:11897497-Animals,
pubmed-meshheading:11897497-Antigens, CD,
pubmed-meshheading:11897497-Antigens, CD19,
pubmed-meshheading:11897497-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:11897497-B-Lymphocytes,
pubmed-meshheading:11897497-Calcium Signaling,
pubmed-meshheading:11897497-Carrier Proteins,
pubmed-meshheading:11897497-Cell Differentiation,
pubmed-meshheading:11897497-Cell Line,
pubmed-meshheading:11897497-Membrane Microdomains,
pubmed-meshheading:11897497-Mice,
pubmed-meshheading:11897497-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:11897497-Phosphoproteins,
pubmed-meshheading:11897497-Phosphorylation,
pubmed-meshheading:11897497-Phosphotyrosine,
pubmed-meshheading:11897497-Receptor Aggregation,
pubmed-meshheading:11897497-Receptors, Antigen, B-Cell,
pubmed-meshheading:11897497-Receptors, IgG,
pubmed-meshheading:11897497-Signal Transduction,
pubmed-meshheading:11897497-Stem Cells,
pubmed-meshheading:11897497-Tumor Cells, Cultured,
pubmed-meshheading:11897497-Type C Phospholipases
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pubmed:year |
2002
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pubmed:articleTitle |
Developmental differences in B cell receptor-induced signal transduction.
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pubmed:affiliation |
Department of Immunology, Loránd Eötvös University, H-1117 Pázmány Péter sétány 1/C, Budapest, Hungary.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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