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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2002-3-22
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pubmed:abstractText |
Bcl-2 is the prototype of a family of genes that prevent apoptosis. However, several reports indicate that Bcl-2 may also act as a cell cycle modulator. In several human tumors, Bcl-2 expression correlates with a more favorable prognosis and lower tumor proliferative activity. We have shown that Bcl-2 expression delays liver tumor development in transgenic mice even when the gene is turned on shortly before the time of tumor development. We hypothesized that Bcl-2 may delay liver tumorigenesis by interfering with hepatocyte proliferation. To test whether Bcl-2 expression may act on hepatocyte replication we studied liver regeneration in Bcl-2 transgenic mice and wild-type littermates. DNA replication was delayed by approximately 8 h in Bcl-2 transgenic mice compared to the timing of the response in wild-type littermates. Cyclin D expression showed no alterations in the regenerating liver of Bcl-2 transgenic mice. In contrast, there was a delay in the expression of p107, cyclin E and in the activity of cyclin E/cdk 2 activity. These results show that Bcl-2 expression delays cell cycle progression in hepatocytes and suggests that it acts at a step involving cyclin E and p107.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1548-55
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11896583-Animals,
pubmed-meshheading:11896583-Cell Cycle,
pubmed-meshheading:11896583-Cell Cycle Proteins,
pubmed-meshheading:11896583-Cells, Cultured,
pubmed-meshheading:11896583-Cyclin-Dependent Kinases,
pubmed-meshheading:11896583-Cyclins,
pubmed-meshheading:11896583-DNA,
pubmed-meshheading:11896583-DNA Replication,
pubmed-meshheading:11896583-Hepatectomy,
pubmed-meshheading:11896583-Hepatocytes,
pubmed-meshheading:11896583-Immunohistochemistry,
pubmed-meshheading:11896583-Kinetics,
pubmed-meshheading:11896583-Liver Regeneration,
pubmed-meshheading:11896583-Male,
pubmed-meshheading:11896583-Mice,
pubmed-meshheading:11896583-Mice, Transgenic,
pubmed-meshheading:11896583-Nuclear Proteins,
pubmed-meshheading:11896583-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:11896583-Retinoblastoma-Like Protein p107
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pubmed:year |
2002
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pubmed:articleTitle |
Bcl-2 expression delays hepatocyte cell cycle progression during liver regeneration.
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pubmed:affiliation |
Department of Pathology, University of Washington, Seattle, Washington, WA 98195-7705, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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