Linked Life Data

11893080

Source:http://linkedlifedata.com/resource/pubmed/id/11893080

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-3-14
pubmed:abstractText
Tubular aggregates are specific subcellular structures that appear in skeletal muscle fibres under different pathological conditions. The origin of the tubular aggregates is generally ascribed to proliferating membranes of sarcoplasmic reticulum. There are, however, histochemical indications for the presence of mitochondrial enzymes in tubular aggregates suggesting contribution of mitochondria to the genesis of tubular aggregates. In this study we used an immunocytochemical detection technique to assess participation of mitochondria and of sarcoplasmic reticulum in derivation of tubular aggregates. The fast skeletal muscle fibres (m. gastrocnemius) of mice bearing the double invalidation for both the mitochondrial and the cytosolic isoforms of creatine kinase (CK), an enzyme involved in energetics of muscle cells, were employed as a model muscle with tubular aggregates (Steeghs et al., Cell 89, 93-103, 1997). Immunogold labelling of the bc1 complex, a specific integral protein of the inner mitochondrial membrane, provided strong signals in both the mitochondria and tubular aggregates but not in other ultrastructural components of muscle fibres. A similar strong immunogold signal was obtained when labelling for SERCA1, a specific enzyme of the sarcoplasmic reticulum membrane, in regions of typical occurrence of the sarcoplasmic reticulum and in tubular aggregates. In double labelling experiments, we found simultaneous labelling of tubular aggregates with both the bc1 and SERCA1 antibodies. It is concluded, that in CK-/- mouse both the inner mitochondrial membrane and the membrane of the sarcoplasmic reticulum participate in the formation of tubular aggregates.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0171-9335
pubmed:author
pubmed:issnType
Print
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
101-6
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:11893080-Animals, pubmed-meshheading:11893080-Calcium-Transporting ATPases, pubmed-meshheading:11893080-Creatine Kinase, pubmed-meshheading:11893080-Disease Models, Animal, pubmed-meshheading:11893080-Immunohistochemistry, pubmed-meshheading:11893080-Male, pubmed-meshheading:11893080-Mice, pubmed-meshheading:11893080-Mice, Knockout, pubmed-meshheading:11893080-Microscopy, Electron, pubmed-meshheading:11893080-Microtubules, pubmed-meshheading:11893080-Mitochondria, Muscle, pubmed-meshheading:11893080-Muscle, Skeletal, pubmed-meshheading:11893080-Muscular Diseases, pubmed-meshheading:11893080-RNA, Untranslated, pubmed-meshheading:11893080-Ribonucleoproteins, Small Cytoplasmic, pubmed-meshheading:11893080-Sarcoplasmic Reticulum, pubmed-meshheading:11893080-Sarcoplasmic Reticulum Calcium-Transporting ATPases
pubmed:year
2002
pubmed:articleTitle
Joint participation of mitochondria and sarcoplasmic reticulum in the formation of tubular aggregates in gastrocnemius muscle of CK-/- mice.
pubmed:affiliation
Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, Slovak Republic.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't