| pubmed-article:11891718 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11891718 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:11891718 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:11891718 | lifeskim:mentions | umls-concept:C0282549 | lld:lifeskim |
| pubmed-article:11891718 | lifeskim:mentions | umls-concept:C0006812 | lld:lifeskim |
| pubmed-article:11891718 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:11891718 | lifeskim:mentions | umls-concept:C0022702 | lld:lifeskim |
| pubmed-article:11891718 | lifeskim:mentions | umls-concept:C2709058 | lld:lifeskim |
| pubmed-article:11891718 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:11891718 | lifeskim:mentions | umls-concept:C1550548 | lld:lifeskim |
| pubmed-article:11891718 | lifeskim:mentions | umls-concept:C1555714 | lld:lifeskim |
| pubmed-article:11891718 | lifeskim:mentions | umls-concept:C1705654 | lld:lifeskim |
| pubmed-article:11891718 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:11891718 | pubmed:dateCreated | 2002-3-13 | lld:pubmed |
| pubmed-article:11891718 | pubmed:abstractText | Duration of apoptosis, from onset to final disintegration of the cell, is often short and variable. The apoptotic index (AI), as a snapshot of a transient event of variable length, does not truly represent incidence of apoptosis in the studied cell population. We recently proposed to estimate the cumulative apoptotic index (CAI) by inducing stathmo-apoptosis. A fluorescent inhibitor of caspases (FLICA) FAM-VAD-FMK is used to arrest the process of apoptosis and thereby prevent cell disintegration. Simultaneously, the arrested/apoptotic cells become FLICA-labeled. In the present study, this approach was applied to measure kinetics of HL-60 cell entrance into apoptosis induced via cell surface death receptor or a mitochondria-initiated pathway. Materials and Methods Cultures of HL-60 cells were treated with either TNF-alpha or camptothecin (CPT) in the absence or constant presence of 10-50 microM FLICA. The CAI was measured at different time points for up to 48 h by flow cytometry. Bivariate analysis of DNA content and cell labeling with FLICA was used to correlate apoptosis with the cell-cycle position. | lld:pubmed |
| pubmed-article:11891718 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11891718 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11891718 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11891718 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11891718 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11891718 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11891718 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11891718 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11891718 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11891718 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11891718 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11891718 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11891718 | pubmed:issn | 0196-4763 | lld:pubmed |
| pubmed-article:11891718 | pubmed:author | pubmed-author:SmolewskiPiot... | lld:pubmed |
| pubmed-article:11891718 | pubmed:author | pubmed-author:Darzynkiewicz... | lld:pubmed |
| pubmed-article:11891718 | pubmed:author | pubmed-author:JohnsonGary... | lld:pubmed |
| pubmed-article:11891718 | pubmed:author | pubmed-author:GrabarekJerzy... | lld:pubmed |
| pubmed-article:11891718 | pubmed:author | pubmed-author:LeeBrian WBW | lld:pubmed |
| pubmed-article:11891718 | pubmed:copyrightInfo | Copyright 2002 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:11891718 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11891718 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:11891718 | pubmed:volume | 47 | lld:pubmed |
| pubmed-article:11891718 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11891718 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11891718 | pubmed:pagination | 143-9 | lld:pubmed |
| pubmed-article:11891718 | pubmed:dateRevised | 2010-12-3 | lld:pubmed |
| pubmed-article:11891718 | pubmed:meshHeading | pubmed-meshheading:11891718... | lld:pubmed |
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| pubmed-article:11891718 | pubmed:meshHeading | pubmed-meshheading:11891718... | lld:pubmed |
| pubmed-article:11891718 | pubmed:meshHeading | pubmed-meshheading:11891718... | lld:pubmed |
| pubmed-article:11891718 | pubmed:meshHeading | pubmed-meshheading:11891718... | lld:pubmed |
| pubmed-article:11891718 | pubmed:meshHeading | pubmed-meshheading:11891718... | lld:pubmed |
| pubmed-article:11891718 | pubmed:meshHeading | pubmed-meshheading:11891718... | lld:pubmed |
| pubmed-article:11891718 | pubmed:meshHeading | pubmed-meshheading:11891718... | lld:pubmed |
| pubmed-article:11891718 | pubmed:meshHeading | pubmed-meshheading:11891718... | lld:pubmed |
| pubmed-article:11891718 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11891718 | pubmed:articleTitle | Kinetics of HL-60 cell entry to apoptosis during treatment with TNF-alpha or camptothecin assayed by the stathmo-apoptosis method. | lld:pubmed |
| pubmed-article:11891718 | pubmed:affiliation | Brander Cancer Research Institute, New York Medical College, 19 Bradhurst Avenue, Suite 2400, Hawthorne, NY 10532, USA. | lld:pubmed |
| pubmed-article:11891718 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11891718 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11891718 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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