Linked Life Data

11891715

Source:http://linkedlifedata.com/resource/pubmed/id/11891715

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-3-13
pubmed:abstractText
The behavior of negatively charged single rhodamine-labeled molecules excited to fluorescence in confocal volume elements has been investigated in oscillating voltage gradients between 1 and 15 kV/cm. The effective charge of rhodamin labeled deoxy-uridine triphosphate (Rh-dUTP) is dependent on the field strength applied and likely is due to differences in the screening by counter ions. It can be shown that the attractive forces generated by an oscillating dipole can increase the concentration of Rh-dUTP by a factor 2. Likewise a substantial increase of concentration can be obtained for Rh-labeled DNA molecules when linear voltage gradients are applied. The importance for diagnostic analysis at the single molecule level is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0173-0835
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
805-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Brownian motion of single molecules in electric fields.
pubmed:affiliation
Department of Medical Biophysics, Karolinska Institutet, Stockholm, Sweden. rudolf.rigler@mbb.ki.se
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't