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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2002-3-13
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pubmed:abstractText |
gamma-Glutamyl transpeptidase (gamma-GT) is a ubiquitous enzyme that catalyzes the first step of glutathione (GSH) degradation in the gamma-glutamyl cycle in mammals. A cDNA encoding an Arabidopsis homolog for gamma-GT was overexpressed in tobacco (Nicotiana tabacum) plants. A high level of the membrane-bound gamma-GT activity was localized outside the cell in transgenic plants. The overproduced enzyme was characterized by a high affinity to GSH and was cleaved post-translationally in two unequal subunits. Thus, Arabidopsis gamma-GT is similar to the mammalian enzymes in enzymatic properties, post-translational processing, and cellular localization, suggesting analogous biological functions as a key enzyme in the catabolism of GSH.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-10490284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-10759537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-10802540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-1128700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-14074151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-1455517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-16453789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-16664906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-1678521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-1706466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-19479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-3016666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-3053703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-4022773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-44433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-6030345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-6122208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-6137189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-6143552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-6143574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-7479844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-8755578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-9139674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-9305623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-942051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-977587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11891265-9872931
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0032-0889
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
128
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1109-19
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:11891265-Amino Acid Sequence,
pubmed-meshheading:11891265-Animals,
pubmed-meshheading:11891265-Arabidopsis,
pubmed-meshheading:11891265-Cell Membrane,
pubmed-meshheading:11891265-Cloning, Molecular,
pubmed-meshheading:11891265-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:11891265-Glutathione,
pubmed-meshheading:11891265-Hydrogen-Ion Concentration,
pubmed-meshheading:11891265-Mammals,
pubmed-meshheading:11891265-Microscopy, Confocal,
pubmed-meshheading:11891265-Molecular Sequence Data,
pubmed-meshheading:11891265-Multigene Family,
pubmed-meshheading:11891265-Oxidative Stress,
pubmed-meshheading:11891265-Plants, Genetically Modified,
pubmed-meshheading:11891265-Sequence Homology, Amino Acid,
pubmed-meshheading:11891265-Tobacco,
pubmed-meshheading:11891265-gamma-Glutamyltransferase
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pubmed:year |
2002
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pubmed:articleTitle |
Gamma-glutamyl transpeptidase in transgenic tobacco plants. Cellular localization, processing, and biochemical properties.
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pubmed:affiliation |
Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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