Source:http://linkedlifedata.com/resource/pubmed/id/11886521
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2002-3-11
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| pubmed:abstractText |
CD8(+) T cell infiltration into the epidermis is thought to be a key event in the pathogenesis of psoriasis. A quantitative competitive polymerase chain reaction method was developed to examine the expression of T cell receptor beta chain variable region 2, 3, 6.1-3, 8, and 13.1 genes in the epidermis of psoriatic lesions. Paired epidermal samples and peripheral blood samples from five psoriasis patients were studied. The results demonstrated the expansion of T cell receptor beta chain variable region 3 (two patients), 8 (two patients), and/or 2 (one patient). Contrary to previous reports, neither beta chain variable region 6.1-3 nor beta chain variable region 13.1 subgroups were expanded in any of the lesions. DNA sequence analysis revealed dominant T cell clones observed in all expanded beta chain variable region families and heterogeneous populations and/or small clones observed in non-expanded beta chain variable region families. Using CDR3 length analysis to examine the complete beta chain repertoire of the infiltrating T cells in the lesional epidermis, we found that approximately 50% of the T cell receptor beta chain variable region families in each patient's lesion demonstrated abnormal CDR3 DNA length distribution, indicating the presence of monoclonal or oligoclonal T cell expansion. Together, the results show that among different patients, T cell oligoclonality is not restricted to a limited number of T cell receptor beta chain variable region families. In an attempt to identify the pathogenic T cells among the many expanded T cell clones in the lesions, we compared T cell receptor expansion in the lesional epidermis with non-lesional epidermis. Particular T cell receptor were found to be preferentially expanded in lesional epidermis and these lesion-specific T cell clones may be most important in the pathogenesis and development of psoriatic lesions.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0022-202X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
117
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1546-53
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| pubmed:dateRevised |
2005-11-17
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| pubmed:meshHeading |
pubmed-meshheading:11886521-Adult,
pubmed-meshheading:11886521-Aged,
pubmed-meshheading:11886521-Amino Acid Sequence,
pubmed-meshheading:11886521-Autoimmune Diseases,
pubmed-meshheading:11886521-Biopsy,
pubmed-meshheading:11886521-CD8-Positive T-Lymphocytes,
pubmed-meshheading:11886521-Clone Cells,
pubmed-meshheading:11886521-Complementarity Determining Regions,
pubmed-meshheading:11886521-Epidermis,
pubmed-meshheading:11886521-Female,
pubmed-meshheading:11886521-Humans,
pubmed-meshheading:11886521-Immunoglobulin Variable Region,
pubmed-meshheading:11886521-Male,
pubmed-meshheading:11886521-Middle Aged,
pubmed-meshheading:11886521-Molecular Sequence Data,
pubmed-meshheading:11886521-Polymerase Chain Reaction,
pubmed-meshheading:11886521-Psoriasis,
pubmed-meshheading:11886521-Receptors, Antigen, T-Cell, alpha-beta
|
| pubmed:year |
2001
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| pubmed:articleTitle |
Oligoclonal expansion of intraepidermal T cells in psoriasis skin lesions.
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| pubmed:affiliation |
Gilead Sciences, Inc., Boulder, Colorado, USA. weijenlin@csupomona.edu
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| pubmed:publicationType |
Journal Article
|