Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-3-11
pubmed:abstractText
The extract of European mistletoe (Viscum album, L) has been used in adjuvant chemotherapy of cancer and mistletoe lectins are considered to be major active components. The present work was performed to investigate the effects of Korean mistletoe lectin (Viscum album L. coloratum agglutinin, VCA) on proliferation and apoptosis of human hepatoma cells as well as the underlying mechamisms for these effects. We showed that VCA induced apoptosis in both SK-Hep-1 (p53-positive) and Hep 3B (p53-negative) cells through p53- and p21-independent pathways. VCA induced apoptosis by down-regulation of Bcl-2 and by up-regulation of Bax functioning upstream of caspase-3 in both cell lines. In addition, we observed down-regulation of telomerase activity in both VCA-treated cells. Our results provide direct evidence of the anti-tumor potential of this biological response which comes from inhibition of telomerase and consequent inducing apoptosis. VCA-induced apoptosis is regulated by mitochondrial controlled pathway independently of p53. These findings are important for the therapy with preparation of mistletoe because they show that telomerase-dependent mechanism can be targeted by VCA in human hepatocarcinoma. Taken together, our results suggest that the VCA, considered as a telomerase-inhibitor, can be envisaged as a candidate for enhancing sensitivity of conventional anticancer drugs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, Phytogenic, http://linkedlifedata.com/resource/pubmed/chemical/BAX protein, human, http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3, http://linkedlifedata.com/resource/pubmed/chemical/Caspases, http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Protein p21(ras), http://linkedlifedata.com/resource/pubmed/chemical/Plant Preparations, http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2, http://linkedlifedata.com/resource/pubmed/chemical/Ribosome Inactivating Proteins..., http://linkedlifedata.com/resource/pubmed/chemical/Telomerase, http://linkedlifedata.com/resource/pubmed/chemical/Toxins, Biological, http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein, http://linkedlifedata.com/resource/pubmed/chemical/ribosome inactivating protein...
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0253-6269
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-101
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:11885700-Antineoplastic Agents, Phytogenic, pubmed-meshheading:11885700-Apoptosis, pubmed-meshheading:11885700-Blotting, Western, pubmed-meshheading:11885700-Carcinoma, Hepatocellular, pubmed-meshheading:11885700-Caspase 3, pubmed-meshheading:11885700-Caspases, pubmed-meshheading:11885700-Cell Nucleus, pubmed-meshheading:11885700-DNA, Neoplasm, pubmed-meshheading:11885700-DNA Fragmentation, pubmed-meshheading:11885700-Down-Regulation, pubmed-meshheading:11885700-Enzyme Inhibitors, pubmed-meshheading:11885700-Flow Cytometry, pubmed-meshheading:11885700-Genes, bcl-2, pubmed-meshheading:11885700-Genes, p53, pubmed-meshheading:11885700-Humans, pubmed-meshheading:11885700-Korea, pubmed-meshheading:11885700-Mitochondria, pubmed-meshheading:11885700-Neoplasm Proteins, pubmed-meshheading:11885700-Oncogene Protein p21(ras), pubmed-meshheading:11885700-Plant Preparations, pubmed-meshheading:11885700-Plant Proteins, pubmed-meshheading:11885700-Proto-Oncogene Proteins, pubmed-meshheading:11885700-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:11885700-Ribosome Inactivating Proteins, Type 2, pubmed-meshheading:11885700-Telomerase, pubmed-meshheading:11885700-Toxins, Biological, pubmed-meshheading:11885700-Tumor Cells, Cultured, pubmed-meshheading:11885700-bcl-2-Associated X Protein
pubmed:year
2002
pubmed:articleTitle
Korean mistletoe lectin-induced apoptosis in hepatocarcinoma cells is associated with inhibition of telomerase via mitochondrial controlled pathway independent of p53.
pubmed:affiliation
College of Natural Sciences, Seoul Women's University, Seoul, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't