Linked Life Data

11884082

Source:http://linkedlifedata.com/resource/pubmed/id/11884082

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-3-8
pubmed:databankReference
pubmed:abstractText
Follicle-stimulating hormone (FSH) is a member of pituitary glycoprotein hormones that are composed of two dissimilar subunits, alpha and beta. Very little information is available regarding the nucleotide and amino acid sequence of FSH-beta in avian species. For better understanding of the phylogenic diversity and evolution of FSH molecule, we have isolated and sequenced the complete complementary DNA (cDNA) encoding chicken FSH-beta precursor molecule by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE) methods. The cloned chicken FSH-beta cDNA consists of 2457-bp nucleotides, including 44-bp nucleotides of the 5'-untranslated region (UTR), 396 bp of the open reading frame, and an extraordinarily long 3'-UTR of 2001-bp nucleotides followed by a poly(A)((16)) tail. It encodes a 131-amino-acid precursor molecule of FSH-beta-subunit with a signal peptide of 20 amino acids followed by a mature protein of 111 amino acids. Twelve cysteine residues, forming six disulfide bonds within beta-subunit and two putative asparagine-linked glycosylation sites, are also conserved in the chicken FSH-beta-subunit. Four proline residues, presumably responsible for changing the backbone direction of protein structure, are conserved in chicken FSH-beta-subunit as well. The nucleotide sequence of chicken FSH-beta cDNA shows high homology with quail FSH-beta cDNA, 97% homology in the open reading frame, and 85% homology in the 3'-UTR. The deduced amino acid sequence of chicken FSH-beta-subunit shows a remarkable similarity to other avian FSH-beta-subunits, 98% homology with quail, and 93% homology with ostrich, whereas a lower similarity (66 to 70%) is noted when compared with mammalian FSH-beta-subunits. By contrast, when comparing with the beta-subunits of chicken luteinizing hormone and thyroid-stimulating hormone, the homologies are as low as 37 and 40%, respectively. FSH-beta mRNA was only expressed in pituitary gland out of various tissues examined and can be up-regulated by gonadotropin-releasing hormone in pituitary tissue culture as estimated by real-time quantitative PCR.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0016-6480
pubmed:author
pubmed:copyrightInfo
(C)2002 Elsevier Science (USA).
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
375-86
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11884082-3' Untranslated Regions, pubmed-meshheading:11884082-5' Untranslated Regions, pubmed-meshheading:11884082-Amino Acid Sequence, pubmed-meshheading:11884082-Animals, pubmed-meshheading:11884082-Chickens, pubmed-meshheading:11884082-Cloning, Molecular, pubmed-meshheading:11884082-DNA, Complementary, pubmed-meshheading:11884082-Follicle Stimulating Hormone, pubmed-meshheading:11884082-Follicle Stimulating Hormone, beta Subunit, pubmed-meshheading:11884082-Gene Expression Regulation, pubmed-meshheading:11884082-Gonadotropin-Releasing Hormone, pubmed-meshheading:11884082-Male, pubmed-meshheading:11884082-Molecular Sequence Data, pubmed-meshheading:11884082-Organ Specificity, pubmed-meshheading:11884082-Phylogeny, pubmed-meshheading:11884082-Pituitary Gland, pubmed-meshheading:11884082-RNA, pubmed-meshheading:11884082-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2002
pubmed:articleTitle
Cloning and gene expression of a cDNA for the chicken follicle-stimulating hormone (FSH)-beta-subunit.
pubmed:affiliation
Endocrinology Laboratory, Institute of Zoology, Academia Sinica, Taipei, 115, Taiwan, Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't