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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2002-3-8
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pubmed:abstractText |
The heterochronic gene lin-28 of the nematode Caenorhabditis elegans controls the relative timing of diverse developmental events during the animal's larval stages. lin-28 is stage-specifically regulated by two genetic circuits: negatively by the 22-nt RNA lin-4 and positively by the heterochronic gene lin-14. Here, we show that lin-28 is repressed during normal development by a mechanism that acts on its mRNA after translation initiation. We provide evidence that lin-14 inhibits a negative regulation that is independent of the lin-4 RNA and involves the gene daf-12, which encodes a nuclear hormone receptor. The lin-4-independent repression does not affect the initiation of translation on the lin-28 mRNA, and like the lin-4-mediated repression, acts through the gene's 3'-untranslated region. In addition, we find that lin-4 is not sufficient to cause repression of lin-28 if the lin-4-independent circuit is inhibited. Therefore, the lin-4-independent circuit likely contributes substantially to the down-regulation of lin-28 that occurs during normal development. The role of lin-4 may be to initiate or potentiate the lin-4-independent circuit. We speculate that a parallel lin-4-independent regulatory mechanism regulates the expression of lin-14.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/DAF-12 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/Helminth Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/LIN-14 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/MicroRNAs,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/lin-4 microRNA, C elegans
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0012-1606
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pubmed:author |
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pubmed:copyrightInfo |
(C)2002 Elsevier Science (USA).
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
243
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
215-25
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11884032-Animals,
pubmed-meshheading:11884032-Animals, Genetically Modified,
pubmed-meshheading:11884032-Caenorhabditis elegans,
pubmed-meshheading:11884032-Caenorhabditis elegans Proteins,
pubmed-meshheading:11884032-Centrifugation, Density Gradient,
pubmed-meshheading:11884032-Cycloheximide,
pubmed-meshheading:11884032-Gene Expression Regulation, Developmental,
pubmed-meshheading:11884032-Helminth Proteins,
pubmed-meshheading:11884032-Larva,
pubmed-meshheading:11884032-MicroRNAs,
pubmed-meshheading:11884032-Models, Genetic,
pubmed-meshheading:11884032-Nuclear Proteins,
pubmed-meshheading:11884032-Peptide Chain Elongation, Translational,
pubmed-meshheading:11884032-Peptide Chain Initiation, Translational,
pubmed-meshheading:11884032-Protein Processing, Post-Translational,
pubmed-meshheading:11884032-Protein Synthesis Inhibitors,
pubmed-meshheading:11884032-RNA, Messenger,
pubmed-meshheading:11884032-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:11884032-Repressor Proteins,
pubmed-meshheading:11884032-Ribosomes
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pubmed:year |
2002
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pubmed:articleTitle |
Two genetic circuits repress the Caenorhabditis elegans heterochronic gene lin-28 after translation initiation.
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pubmed:affiliation |
Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA.
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pubmed:publicationType |
Journal Article
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