|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0021760,
umls-concept:C0036770,
umls-concept:C0079189,
umls-concept:C0205101,
umls-concept:C0871261,
umls-concept:C1515655,
umls-concept:C1522449,
umls-concept:C1533691,
umls-concept:C1552644,
umls-concept:C1554080,
umls-concept:C1704632,
umls-concept:C1706198,
umls-concept:C1706817,
umls-concept:C1823153,
umls-concept:C2349976,
umls-concept:C2911692
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
2002-3-8
|
|
pubmed:abstractText |
Interleukin 1(IL-1) and IL-6 are cytokines involved in the response to radiation and are known for their radioprotective properties with respect to total-body irradiation. We previously showed that after gamma irradiation of Sertoli cells (SC), we observed an increase in the activity of IL-6 but not of IL-1. The aim of this study was to see whether this response is a function of the differentiation of SC, to analyse the mechanisms responsible for this induction, and to test whether this cytokine has a radioprotective role on germ cells. Unlike IL-1, a dose-dependent increase of IL-6 activity in SC following gamma irradiation at high doses was observed at all ages studied. On the other hand, radio-induction observed at low doses (<1Gy) was dose-independent. IL-6 up-regulation resulted from transcriptional activation as shown by the use of specific inhibitors. The injection of IL-1 and IL-6 in mice prior to whole-body irradiation resulted in an increased survival rate. Moreover, cytokines protected DNA from remaining cells following irradiation as shown by comet assay on germ cells. In conclusion, IL-6 seems to constitute a good marker of exposure to gamma irradiation, both at low and high doses. In addition, we showed that IL-1 and IL-6 have a radioprotective effect at testicular level.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
1043-4666
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright 2001 Academic Press.
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
21
|
|
pubmed:volume |
16
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
232-8
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:11884027-Animals,
pubmed-meshheading:11884027-Cell Survival,
pubmed-meshheading:11884027-Comet Assay,
pubmed-meshheading:11884027-Cycloheximide,
pubmed-meshheading:11884027-Dactinomycin,
pubmed-meshheading:11884027-Dose-Response Relationship, Radiation,
pubmed-meshheading:11884027-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11884027-Female,
pubmed-meshheading:11884027-Humans,
pubmed-meshheading:11884027-Interleukin-1,
pubmed-meshheading:11884027-Interleukin-6,
pubmed-meshheading:11884027-Male,
pubmed-meshheading:11884027-Mice,
pubmed-meshheading:11884027-Mice, Inbred C3H,
pubmed-meshheading:11884027-Protein Synthesis Inhibitors,
pubmed-meshheading:11884027-Radiation-Sensitizing Agents,
pubmed-meshheading:11884027-Rats,
pubmed-meshheading:11884027-Rats, Sprague-Dawley,
pubmed-meshheading:11884027-Recombinant Proteins,
pubmed-meshheading:11884027-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11884027-Sertoli Cells,
pubmed-meshheading:11884027-Testis,
pubmed-meshheading:11884027-Time Factors
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
IL-6 a key cytokine in in vitro and in vivo response of Sertoli cells to external gamma irradiation.
|
|
pubmed:affiliation |
UPRES EA 2231, Université de Rennes I, Centre Eugène Marquis, CS 44229, 35042 Rennes Cedex, France.
|
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|
