Linked Life Data

11882476

Source:http://linkedlifedata.com/resource/pubmed/id/11882476

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-3-7
pubmed:abstractText
Earlier studies have shown that air/blood partition coefficients (PCs) for many volatile organic chemicals (VOCs) are much higher in rat blood than in human blood. It has been suggested that the discrepancy could be attributed to the fact that hemoglobin (Hb) in rat blood exists in a quasi-crystalline form of hydrophobicity greater than that of normal human Hb (HbA) and thus has a higher carrying capacity for VOCs. In the present study, we used benzene as a prototypic VOC to examine its relative partitioning into human and animal blood. Additionally, we sought to ascertain whether the water-insoluble form of hemoglobin (HbS) found in subjects with homozygous sickle cell (SC) disease has a greater VOC-carrying capacity than does HbA blood. At a low-O(2) tension, HbS switches to water-insoluble polymers, which physically deforms the red blood cells (RBCs) to the sickle shape. We equilibrated HbA, HbS, Hartley guinea pig, CD1 mouse, and rat (F-344, Wistar, and Sprague-Dawley) blood and their respective fractions with benzene vapor (80 or 400 ppm) for 3 hr at 37 degrees C in air-tight vials. We introduced benzene vapor into the vial head space that contained air or respiratory mixtures of venous-type (low-O(2)) or arterial-type (high-O(2)) gases. The blood measurements included the PC, Hb, partial pressures of O(2)(pO(2)) and CO(2)(pCO(2), pH, and percentage of SCs. The benzene concentration had no effect on these parameters, and the high- and low-O(2) gas mixtures produced the expected changes in pO(2), pCO(2), and pH. At equilibrium, the low-O(2) HbS blood had approximately 85% SCs compared with roughly 15% with air or high-O(2) gas. PCs for rat and mouse blood were about 100% higher than those for human and guinea pig blood, but the PC for deoxygenated HbS blood was only slightly higher than that for HbA or oxygenated HbS blood. Benzene showed higher affinities for RBCs in the deoxygenated HbS, rat, and mouse blood and higher affinity for plasma in the guinea pig blood. There was no evidence of disproportionate partitioning of benzene into oxygenated HbS or into HbA blood forms. These data suggest that the water solubility of Hb alone appears to have little effect on the VOC-carrying capacity of blood and that the influence of species is large in comparison. These latter differences in partitioning may depend on the number of hydrophobic sites on the surface of the plasma/heme proteins and thus be unique to the species.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11882476-1442788, http://linkedlifedata.com/resource/pubmed/commentcorrection/11882476-2360202, http://linkedlifedata.com/resource/pubmed/commentcorrection/11882476-2435321, http://linkedlifedata.com/resource/pubmed/commentcorrection/11882476-2477239, http://linkedlifedata.com/resource/pubmed/commentcorrection/11882476-2929023, http://linkedlifedata.com/resource/pubmed/commentcorrection/11882476-3176068, http://linkedlifedata.com/resource/pubmed/commentcorrection/11882476-3656444, http://linkedlifedata.com/resource/pubmed/commentcorrection/11882476-4710369, http://linkedlifedata.com/resource/pubmed/commentcorrection/11882476-4782530, http://linkedlifedata.com/resource/pubmed/commentcorrection/11882476-500783, http://linkedlifedata.com/resource/pubmed/commentcorrection/11882476-6396052, http://linkedlifedata.com/resource/pubmed/commentcorrection/11882476-8314465
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0091-6765
pubmed:author
pubmed:issnType
Print
pubmed:volume
110
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
255-61
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Partitioning of benzene in blood: influence of hemoglobin type in humans and animals.
pubmed:affiliation
Office of Research and Development, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711, USA. wiester.mildred@epa.gov
pubmed:publicationType
Journal Article, Comparative Study