Linked Life Data

1188150

Source:http://linkedlifedata.com/resource/pubmed/id/1188150

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1976-2-2
pubmed:abstractText
Using both isotopic and spectrophotometric techniques to monitor the overall Ca++ binding and release reactions, we have studied the effects of the relative free concentrations of Ca++, sarcoplasmic reticulum fragments (SRF) protein, and ATP on spontaneous Ca++ release by SRF isolated from normal canine hearts. Low free [Ca++] was achieved by using a Ca-EGTA buffer; higher (micromolar) values were obtained by direct Ca++ additions throughout the reactions. ATP concentrations were stabilized by an ATP-regenerating system or an ATP trap. Concentrations of nucleotides in the reaction mixtures were also determined. Release depended in part on the [Ca++]/[ATP] and on the [ATP]. Spontaneous Ca++ release was prevented or slowed when the [ATP] was rapidly lowered with the ATP trap. ATP regeneration delayed, but did not prevent Ca++ release. Ca++-induced Ca++ release was found in the presence of ADP and ATP. Verapamil and D 600 did not affect release. The results may provide a basis for the slowed Ca++ release in SRF from failing hearts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0363-5872
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
143-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Role of free calcium and ATP in calcium release from cardiac sarcoplasmic reticulum fragments.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.