Linked Life Data

11880394

Source:http://linkedlifedata.com/resource/pubmed/id/11880394

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-3-6
pubmed:abstractText
We have evaluated the VERSANT HCV RNA 3.0. Assay (HCV 3.0 bDNA assay) (Bayer Diagnostics, Berkeley, Calif.), which is an improved signal amplification procedure for the HCV 2.0 bDNA assay for the quantitation of hepatitis C virus (HCV) RNA in serum or plasma of HCV-infected individuals. The HCV 3.0 bDNA assay has a linear dynamic range of 2.5 x 10(3) to 4.0 x 10(7) HCV RNA copies per ml (c/ml). The performance of the HCV 3.0 bDNA assay was evaluated using three different test panels. An overall specificity of 96.8% relative to the detection limit of the HCV 3.0 bDNA assay was found. The intra- and interrun reproducibilities for both the dilution panel and the NAP (AcroMetrix, Benicia, Calif.) panel were consistent with coefficients of variation of less than 9%. Quantitation with the HCV 3.0 bDNA assay was linear over the entire range of both panels (ranges of 4.4 x 10(3) to 3.5 x 10(6) c/ml and 5 x 10(3) to 2 x 10(6) IU/ml, respectively), with correlation coefficients of 0.999, slopes close to one, and intercepts close to zero. The regression equation indicated that 1 IU corresponded to about 4.8 copies of HCV RNA. A correlation coefficient of 0.941 was found for HCV RNA values (in international units per milliliter) obtained from the HCV 3.0 bDNA assay and the HCV Monitor version 2.0 assay (HCV Monitor 2.0 assay) (Roche Diagnostic Systems, Branchburg, N.J.). Quantitative results obtained close to the lower limit of the HCV 3.0 bDNA assay might imply that its lower limit should be reconsidered and raised, if necessary. It appeared that quantitation values obtained from the HCV Monitor 2.0 assay of between 5 x 10(2) and 10(5) IU/ml were in general higher than those obtained from the HCV 3.0 bDNA assay, whereas values obtained from the HCV Monitor 2.0 assay were underestimated for samples with HCV RNA levels above 10(5) IU/ml.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11880394-10341329, http://linkedlifedata.com/resource/pubmed/commentcorrection/11880394-10381092, http://linkedlifedata.com/resource/pubmed/commentcorrection/11880394-10405395, http://linkedlifedata.com/resource/pubmed/commentcorrection/11880394-10622553, http://linkedlifedata.com/resource/pubmed/commentcorrection/11880394-10960464, http://linkedlifedata.com/resource/pubmed/commentcorrection/11880394-11060086, http://linkedlifedata.com/resource/pubmed/commentcorrection/11880394-11106715, http://linkedlifedata.com/resource/pubmed/commentcorrection/11880394-11163581, http://linkedlifedata.com/resource/pubmed/commentcorrection/11880394-8681488, http://linkedlifedata.com/resource/pubmed/commentcorrection/11880394-9756471, http://linkedlifedata.com/resource/pubmed/commentcorrection/11880394-9807989, http://linkedlifedata.com/resource/pubmed/commentcorrection/11880394-9819446
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
788-93
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Performance of the New Bayer VERSANT HCV RNA 3.0 assay for quantitation of hepatitis C virus RNA in plasma and serum: conversion to international units and comparison with the Roche COBAS Amplicor HCV Monitor, Version 2.0, assay.
pubmed:affiliation
Section of Clinical Virology, Department of Medical Microbiology, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands. m.beld@amc.uva.nl
pubmed:publicationType
Journal Article, Comparative Study