Linked Life Data

11879578

Source:http://linkedlifedata.com/resource/pubmed/id/11879578

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-3-6
pubmed:abstractText
The serum amyloid A activating factor (SAF) was identified as a family of inducible transcription factors that is activated by many mediators of inflammation. Its activation involves a phosphorylation event, whose mechanism is not fully understood. Here, we show that cAMP treatment of several cell types, including mouse liver-derived BNL CL.2, human monocyte-derived THP-1, and a primary culture of vascular smooth muscle cells from porcine aorta, activated cellular SAF's ability to bind DNA. The protein kinase A (PKA) activity in cytoplasmic extracts of cAMP-treated cells was responsible for the potentiation of the DNA-binding activity of the cellular SAF proteins. Furthermore, treatment of nuclear extracts of untreated cells with purified PKA increased the DNA-binding activity of cellular SAF proteins, and specific inhibitors of PKA abrogated the enhanced DNA-binding ability of SAF in the cAMP-treated cells. Consistent with these findings, overexpression of the catalytic subunit of PKA markedly increased expression of the SAF-regulated promoter. These results imply a functional role for the previously detected protein-protein interaction between SAF-1 transcription factor and the catalytic subunit of PKA and further demonstrate the consequences of cAMP-mediated signaling for the expression of SAF-regulated genes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1044-5498
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
31-40
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:11879578-8-Bromo Cyclic Adenosine Monophosphate, pubmed-meshheading:11879578-Animals, pubmed-meshheading:11879578-Blotting, Western, pubmed-meshheading:11879578-Cell Line, pubmed-meshheading:11879578-Cyclic AMP, pubmed-meshheading:11879578-Cyclic AMP-Dependent Protein Kinases, pubmed-meshheading:11879578-DNA, pubmed-meshheading:11879578-DNA Primers, pubmed-meshheading:11879578-DNA-Binding Proteins, pubmed-meshheading:11879578-Electrophoretic Mobility Shift Assay, pubmed-meshheading:11879578-Enzyme Inhibitors, pubmed-meshheading:11879578-Gene Expression Regulation, pubmed-meshheading:11879578-Monocytes, pubmed-meshheading:11879578-Muscle, Smooth, Vascular, pubmed-meshheading:11879578-Signal Transduction, pubmed-meshheading:11879578-Swine, pubmed-meshheading:11879578-Transcription Factors
pubmed:year
2002
pubmed:articleTitle
Promoter-binding activity of inflammation-responsive transcription factor SAF is regulated by cyclic AMP signaling pathway.
pubmed:affiliation
Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri 65211, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't