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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2002-3-5
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pubmed:abstractText |
Local gene therapy has enormous potential for the treatment of vascular disease. We determined whether diagnostic ultrasound-mediated destruction of plasmid-loaded albumin microbubbles is a feasible and efficient technique for local vascular gene delivery. For gene transfer, we used a phosphomimetic, active endothelial nitric oxide synthase (eNOS) construct in which Ser1177 was replaced by aspartic acid (S1177D) and exhibits a 2-fold higher basal activity than the wild-type enzyme.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1524-4539
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
5
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pubmed:volume |
105
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1104-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11877363-Amino Acid Substitution,
pubmed-meshheading:11877363-Animals,
pubmed-meshheading:11877363-Blotting, Western,
pubmed-meshheading:11877363-Coronary Vessels,
pubmed-meshheading:11877363-Dinoprost,
pubmed-meshheading:11877363-Drug Delivery Systems,
pubmed-meshheading:11877363-Echocardiography,
pubmed-meshheading:11877363-Endothelium, Vascular,
pubmed-meshheading:11877363-Enzyme Inhibitors,
pubmed-meshheading:11877363-Feasibility Studies,
pubmed-meshheading:11877363-Gene Therapy,
pubmed-meshheading:11877363-Gene Transfer Techniques,
pubmed-meshheading:11877363-Genes, Reporter,
pubmed-meshheading:11877363-Nitric Oxide Synthase,
pubmed-meshheading:11877363-Nitric Oxide Synthase Type III,
pubmed-meshheading:11877363-Plasmids,
pubmed-meshheading:11877363-Serum Albumin,
pubmed-meshheading:11877363-Sonication,
pubmed-meshheading:11877363-Swine,
pubmed-meshheading:11877363-Transfection,
pubmed-meshheading:11877363-Ultrasonography, Interventional,
pubmed-meshheading:11877363-Vasomotor System,
pubmed-meshheading:11877363-beta-Galactosidase
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pubmed:year |
2002
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pubmed:articleTitle |
Vascular gene transfer of phosphomimetic endothelial nitric oxide synthase (S1177D) using ultrasound-enhanced destruction of plasmid-loaded microbubbles improves vasoreactivity.
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pubmed:affiliation |
Molecular Cardiology, Department of Medicine IV, Institut für Kardiovaskuläre Physiologie, University of Frankfurt, Germany.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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