Source:http://linkedlifedata.com/resource/pubmed/id/11875502
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2002-3-4
|
| pubmed:abstractText |
We constructed miniaturized autoantigen arrays to perform large-scale multiplex characterization of autoantibody responses directed against structurally diverse autoantigens, using submicroliter quantities of clinical samples. Autoantigen microarrays were produced by attaching hundreds of proteins, peptides and other biomolecules to the surface of derivatized glass slides using a robotic arrayer. Arrays were incubated with patient serum, and spectrally resolvable fluorescent labels were used to detect autoantibody binding to specific autoantigens on the array. We describe and characterize arrays containing the major autoantigens in eight distinct human autoimmune diseases, including systemic lupus erythematosus and rheumatoid arthritis. This represents the first report of application of such technology to multiple human disease sera, and will enable validated detection of antibodies recognizing autoantigens including proteins, peptides, enzyme complexes, ribonucleoprotein complexes, DNA and post-translationally modified antigens. Autoantigen microarrays represent a powerful tool to study the specificity and pathogenesis of autoantibody responses, and to identify and define relevant autoantigens in human autoimmune diseases.
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| pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/5R01NS18235,
http://linkedlifedata.com/resource/pubmed/grant/K08 AI01521,
http://linkedlifedata.com/resource/pubmed/grant/K08 AR02133,
http://linkedlifedata.com/resource/pubmed/grant/U19 DK61934,
http://linkedlifedata.com/resource/pubmed/grant/U19DK61934
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1078-8956
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| pubmed:author |
pubmed-author:BrownPatrick OPO,
pubmed-author:DeanErik JEJ,
pubmed-author:DiGennaroCarlaC,
pubmed-author:FongDerekD,
pubmed-author:FournelSylvieS,
pubmed-author:GenoveseMark CMC,
pubmed-author:HaabBrian BBB,
pubmed-author:HirschbergDavid LDL,
pubmed-author:HueberWolfgangW,
pubmed-author:KamachiMakotoM,
pubmed-author:MorrisRobert IRI,
pubmed-author:MullerSylvianeS,
pubmed-author:PruijnGer JGJ,
pubmed-author:RobinsonWilliam HWH,
pubmed-author:SkrinerKarlK,
pubmed-author:SmolenJosef SJS,
pubmed-author:SteinmanLawrenceL,
pubmed-author:UtzPaul JPJ,
pubmed-author:de VegvarHenry E NeumanHE,
pubmed-author:van VenrooijWalther JWJ
|
| pubmed:issnType |
Print
|
| pubmed:volume |
8
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
295-301
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:11875502-Animals,
pubmed-meshheading:11875502-Autoantibodies,
pubmed-meshheading:11875502-Autoantigens,
pubmed-meshheading:11875502-Autoimmune Diseases,
pubmed-meshheading:11875502-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11875502-Fluorescent Dyes,
pubmed-meshheading:11875502-Humans,
pubmed-meshheading:11875502-Immunoglobulin Isotypes,
pubmed-meshheading:11875502-Immunosorbent Techniques,
pubmed-meshheading:11875502-Reproducibility of Results,
pubmed-meshheading:11875502-Sensitivity and Specificity
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
Autoantigen microarrays for multiplex characterization of autoantibody responses.
|
| pubmed:affiliation |
Division of Immunology and Rheumatology, Department of Medicine, Stanford, California, USA. wrobins@stanford.edu
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|