Linked Life Data

11872640

Source:http://linkedlifedata.com/resource/pubmed/id/11872640

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-3-1
pubmed:abstractText
We previously demonstrated that fasting/refeeding enhances the initiation phase of liver and colorectal carcinogenesis in rats. The present study was undertaken to establish whether cycles of fasting/refeeding carried out during the promotion phase of carcinogenesis may also affect the formation of aberrant crypt foci (ACF), preneoplastic lesions induced in the colon by azoxymethane (AOM). We were also interested in studying whether this effect might be mediated by changes in the proliferation, apoptosis or expression of TGFbeta1 and p21CIP genes in the colon. 44 male Fisher 344 rats were given a single dose of AOM (20 mg/kg s.c.) and one week later, they were exposed to 5 cycles of 4 days fasting followed by 7-10 days of refeeding (refed rats); controls were regularly fed; the rats were killed 2, 8 or 30 days after the last cycle of fasting. Fasting/refeeding caused a dramatic increase in crypt multiplicity when compared with regularly fed rats (AC/ACF was 4.30 +/- 1.3 in refed and 2.38 +/- 0.4 in regularly fed rats, P < 0.005 means +/- SD), while no significant changes were observed in the number of ACF/colon. In the two experimental groups, cell proliferation was higher in ACF than in the surrounding mucosa, but proliferative indexes were higher and the apoptotic index lower in ACF of refed rats compared with regularly fed rats. TGFbeta1 expression was higher in the ACF of refed rats than in those of fully fed controls while p21CIP was less expressed in refed rats than in controls. These results suggest that fasting/refeeding is a risk factor for colon cancer and must be taken into account for cancer prevention in humans.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0143-3334
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
323-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11872640-Animals, pubmed-meshheading:11872640-Azoxymethane, pubmed-meshheading:11872640-Carcinogens, pubmed-meshheading:11872640-Cell Division, pubmed-meshheading:11872640-Colon, pubmed-meshheading:11872640-Cyclin-Dependent Kinase Inhibitor p21, pubmed-meshheading:11872640-Cyclins, pubmed-meshheading:11872640-Food Deprivation, pubmed-meshheading:11872640-Humans, pubmed-meshheading:11872640-Immunohistochemistry, pubmed-meshheading:11872640-In Situ Nick-End Labeling, pubmed-meshheading:11872640-Kinetics, pubmed-meshheading:11872640-Male, pubmed-meshheading:11872640-Mucous Membrane, pubmed-meshheading:11872640-Precancerous Conditions, pubmed-meshheading:11872640-Rats, pubmed-meshheading:11872640-Rats, Inbred F344, pubmed-meshheading:11872640-Time Factors, pubmed-meshheading:11872640-Transforming Growth Factor beta, pubmed-meshheading:11872640-Transforming Growth Factor beta1
pubmed:year
2002
pubmed:articleTitle
Enhanced growth of colorectal aberrant crypt foci in fasted/refed rats involves changes in TGFbeta1 and p21CIP expressions.
pubmed:affiliation
Dipartimento di Farmacologia Preclinica e Clinica, Università degli Studi di Firenze, Firenze, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't