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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2002-3-7
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pubmed:abstractText |
The expression of the beta-like globin genes is intricately regulated by a series of both general and tissue-restricted transcription factors. The hemapoietic lineage-specific transcription factor GATA-1 is important for erythroid differentiation and has been implicated in regulating the expression of the erythroid-specific genes including the genes of the beta-globin locus. In the human erythroleukemic K562 cell line, only one DNA region has been identified previously as a putative site of GATA-1 interaction by in vivo footprinting studies. We mapped GATA-1 binding throughout the beta-globin locus by using chIp-chip analysis of K562 cells. We found that GATA-1 binds in a region encompassing the HS2 core element, as was previously identified, and an additional region of GATA-1 binding upstream of the gammaG gene. This approach will be of general utility for mapping transcription factor binding sites within the beta-globin locus and throughout the genome.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-10207073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-10721987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-11125145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-11206552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-11287613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-12073330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-1631062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-1638017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-1924329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-1946439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-2276623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-3456593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-3879975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-7568185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-7828675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-7903220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-7926723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-8400240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-8608018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-8662960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-8901585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-9032291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-9230307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11867748-9482838
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2924-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11867748-Animals,
pubmed-meshheading:11867748-Base Sequence,
pubmed-meshheading:11867748-Binding Sites,
pubmed-meshheading:11867748-Chromatin,
pubmed-meshheading:11867748-Chromosome Mapping,
pubmed-meshheading:11867748-Consensus Sequence,
pubmed-meshheading:11867748-DNA-Binding Proteins,
pubmed-meshheading:11867748-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:11867748-GATA1 Transcription Factor,
pubmed-meshheading:11867748-Globins,
pubmed-meshheading:11867748-HeLa Cells,
pubmed-meshheading:11867748-Humans,
pubmed-meshheading:11867748-K562 Cells,
pubmed-meshheading:11867748-Mammals,
pubmed-meshheading:11867748-Molecular Sequence Data,
pubmed-meshheading:11867748-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:11867748-Precipitin Tests,
pubmed-meshheading:11867748-Transcription Factors
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pubmed:year |
2002
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pubmed:articleTitle |
GATA-1 binding sites mapped in the beta-globin locus by using mammalian chIp-chip analysis.
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pubmed:affiliation |
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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