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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2002-2-27
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pubmed:abstractText |
4-1BB (CDw137) and its ligand (4-1BBL) have been implicated in cellular immune responses. To further characterize the expression and function of 4-1BBL, we newly generated an anti-mouse 4-1BBL mAb (TKS-1), which can inhibit the interaction of 4-1BBL with 4-1BB. Flow cytometric analyses using TKS-1 and an anti-mouse 4-1BB mAb indicated that 4-1BB was inducible on both CD4(+) and CD8(+) splenic T cells by stimulation with immobilized anti-CD3 mAb, but 4-1BBL was not expressed on resting or activated T cells. 4-1BBL expression was inducible on splenic B cells by stimulation with anti-IgM antibody plus anti-CD40 mAb, on peritoneal macrophages by stimulation with lipopolysaccharide (LPS) and on splenic dendritic cells (DC) by stimulation with anti-CD40 mAb or LPS. Interestingly, splenic DC expressed 4-1BB constitutively, which was down-regulated by anti-CD40 stimulation. Co-culture of splenic DC with 4-1BBL-transfected cells or 4-1BBL-expressing tumor cell lines led to cytokine (IL-6 and IL-12) production and co-stimulatory molecule up-regulation by splenic DC, indicating that 4-1BBL can directly activate DC. Moreover, IL-12 production by anti-CD40-stimulated DC was partially inhibited by TKS-1. These results suggest that 4-1BB expressed on DC may be involved in DC activation through DC--tumor interaction and DC--DC interaction.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-1BB Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD137,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfrsf9 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfsf9 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0953-8178
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
275-86
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11867564-4-1BB Ligand,
pubmed-meshheading:11867564-Animals,
pubmed-meshheading:11867564-Antibodies, Monoclonal,
pubmed-meshheading:11867564-Antigens, CD,
pubmed-meshheading:11867564-Antigens, CD137,
pubmed-meshheading:11867564-Antigens, CD40,
pubmed-meshheading:11867564-B-Lymphocytes,
pubmed-meshheading:11867564-Cells, Cultured,
pubmed-meshheading:11867564-Dendritic Cells,
pubmed-meshheading:11867564-Female,
pubmed-meshheading:11867564-Interleukin-12,
pubmed-meshheading:11867564-Macrophages,
pubmed-meshheading:11867564-Male,
pubmed-meshheading:11867564-Mice,
pubmed-meshheading:11867564-Mice, Inbred BALB C,
pubmed-meshheading:11867564-Mice, Inbred C57BL,
pubmed-meshheading:11867564-Mice, Inbred DBA,
pubmed-meshheading:11867564-Mice, Inbred Strains,
pubmed-meshheading:11867564-Mice, Transgenic,
pubmed-meshheading:11867564-Receptors, Nerve Growth Factor,
pubmed-meshheading:11867564-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:11867564-T-Lymphocytes,
pubmed-meshheading:11867564-Tumor Necrosis Factor-alpha
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pubmed:year |
2002
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pubmed:articleTitle |
Expression and function of 4-1BB and 4-1BB ligand on murine dendritic cells.
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pubmed:affiliation |
Department of Immunology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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