Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-2-25
pubmed:abstractText
Wnt ligands and Frizzled (Fz) G protein-coupled receptors impact cell fate, including embryonic development of the ovary. Because the role of these regulatory molecules during follicular development in the adult is not known, an RT-PCR survey was done. Wnt-4, Fz-4, and Fz-1 were among the transcripts detected, and each exhibited a specific pattern of expression. Fz-1 mRNA was low in preovulatory follicles of PMSG-treated mice but was increased within 4-12 h after an ovulatory surge of human CG. By in situ analysis, Fz-1 transcripts increased first in the theca cells and then in the granulosa cells of ovulating follicles but were low in corpora lutea. In contrast, Wnt-4, a critical factor in early ovarian development, was expressed in small preantral follicles. In addition, Wnt-4 was detected in preovulatory follicles and exhibited high levels in corpora lutea. A potential receptor for Wnt-4 in corpora lutea is Fz-4 that was also elevated in this tissue. Although Wnt-4 has been shown to function downstream of the PR in other tissues, Wnt-4 was not altered in follicles of PR-null mice that fail to ovulate. Rather expression of Fz-1 was lower in ovaries of PR knockout mice, compared with normal littermates. Thus, specific Wnt/Fz are expressed at distinct stages of follicular development, suggesting multiple functions for this signaling pathway in the ovary.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers, http://linkedlifedata.com/resource/pubmed/chemical/FZD1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Frizzled Receptors, http://linkedlifedata.com/resource/pubmed/chemical/Fzd1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Fzd1 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Fzd4 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Ligands, http://linkedlifedata.com/resource/pubmed/chemical/Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neurotransmitter, http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Zebrafish Proteins
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
143
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
898-908
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11861511-Animals, pubmed-meshheading:11861511-Blotting, Western, pubmed-meshheading:11861511-Corpus Luteum, pubmed-meshheading:11861511-DNA Primers, pubmed-meshheading:11861511-Female, pubmed-meshheading:11861511-Frizzled Receptors, pubmed-meshheading:11861511-Granulosa Cells, pubmed-meshheading:11861511-In Situ Hybridization, pubmed-meshheading:11861511-Ligands, pubmed-meshheading:11861511-Mice, pubmed-meshheading:11861511-Mice, Inbred C57BL, pubmed-meshheading:11861511-Mice, Knockout, pubmed-meshheading:11861511-Ovarian Follicle, pubmed-meshheading:11861511-Ovary, pubmed-meshheading:11861511-Protein Biosynthesis, pubmed-meshheading:11861511-Proteins, pubmed-meshheading:11861511-Proto-Oncogene Proteins, pubmed-meshheading:11861511-RNA, Messenger, pubmed-meshheading:11861511-Rats, pubmed-meshheading:11861511-Rats, Sprague-Dawley, pubmed-meshheading:11861511-Receptors, Cell Surface, pubmed-meshheading:11861511-Receptors, G-Protein-Coupled, pubmed-meshheading:11861511-Receptors, Neurotransmitter, pubmed-meshheading:11861511-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11861511-Transcription, Genetic, pubmed-meshheading:11861511-Wnt Proteins, pubmed-meshheading:11861511-Zebrafish Proteins
pubmed:year
2002
pubmed:articleTitle
Regulated expression of Wnts and Frizzleds at specific stages of follicular development in the rodent ovary.
pubmed:affiliation
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.