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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2002-2-22
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pubmed:abstractText |
Lateral mobility and spatial organization of proteins within the plasma membrane are likely to mediate the initial events coordinating T cell activation. Lipid rafts, distinct cholesterol/sphingolipid-rich membrane microdomains, provide a mechanism for this regulation by concentrating or excluding signaling proteins. We demonstrate in peripheral blood T cell lymphoblasts that immediate early phosphotyrosine signal transduction through the TCR complex is functionally dependent on a distinct population of lipid rafts. Specifically, cholesterol extraction destabilizes the membrane microdomains containing Lck, while the rafts containing the adapter protein linker for activation of T cells remain intact. Heterogeneity in the partitioning of these proteins in resting cells was confirmed by immunoelectron microscopy. After T cell activation, both Lck and the linker for activation of T cells colocalize to 50-100 nm microdomains in the plasma membrane, indicating that sequestration of these proteins into distinct lipid rafts may function to regulate the initiation of T cell signal transduction.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclodextrins,
http://linkedlifedata.com/resource/pubmed/chemical/LAT protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Specific Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/ZAP-70 Protein-Tyrosine Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/ZAP70 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/antigen T cell receptor, zeta chain,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Cyclodextrins,
http://linkedlifedata.com/resource/pubmed/chemical/methyl-beta-cyclodextrin
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
168
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2233-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11859110-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:11859110-Antigens, CD4,
pubmed-meshheading:11859110-Blood,
pubmed-meshheading:11859110-Carrier Proteins,
pubmed-meshheading:11859110-Cells, Cultured,
pubmed-meshheading:11859110-Cholesterol,
pubmed-meshheading:11859110-Cyclodextrins,
pubmed-meshheading:11859110-Humans,
pubmed-meshheading:11859110-Lymphocyte Activation,
pubmed-meshheading:11859110-Lymphocyte Specific Protein Tyrosine Kinase p56(lck),
pubmed-meshheading:11859110-Membrane Microdomains,
pubmed-meshheading:11859110-Membrane Proteins,
pubmed-meshheading:11859110-Phosphoproteins,
pubmed-meshheading:11859110-Phosphorylation,
pubmed-meshheading:11859110-Phosphotyrosine,
pubmed-meshheading:11859110-Protein-Tyrosine Kinases,
pubmed-meshheading:11859110-Receptors, Antigen, T-Cell,
pubmed-meshheading:11859110-Signal Transduction,
pubmed-meshheading:11859110-T-Lymphocytes,
pubmed-meshheading:11859110-ZAP-70 Protein-Tyrosine Kinase,
pubmed-meshheading:11859110-beta-Cyclodextrins
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pubmed:year |
2002
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pubmed:articleTitle |
Lipid raft heterogeneity in human peripheral blood T lymphoblasts: a mechanism for regulating the initiation of TCR signal transduction.
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pubmed:affiliation |
Department of Pathology, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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