Linked Life Data

11859109

Source:http://linkedlifedata.com/resource/pubmed/id/11859109

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-2-22
pubmed:abstractText
Because the chemokine receptor CCR5 is expressed on Th1 CD4(+) cells, it is important to investigate the expression and function of this receptor on other T cells involved in Th1 immune responses, such as Ag-specific CD8(+) T cells, which to date have been only partially characterized. Therefore, we analyzed the expression and function of CCR5 on virus-specific CD8+ T cells identified by HLA class I tetramers. Multicolor flow cytometry analysis demonstrated that CCR5 is expressed on memory (CD28+CD45RA-) and effector (CD28-CD45RA- and CD28-CD45RA+) CD8+ T cells but not on naive (CD28+CD45RA+) CD8+ T cells. CCR5 expression was much lower on two effector CD8+ T cells than on memory CD8+ T cells. Analysis of CCR7 and CCR5 expression on the different types of CD8+ T cells showed that memory CD8+ T cells have three phenotypic subsets, CCR5+CCR7-, CCR5+CCR7+, and CCR5-CCR7+, while naive and effector CD8+ T cells have CCR5-CCR7+ and CCR5+CCR7- phenotypes, respectively. These results suggest the following sequence for differentiation of memory CD8+ T cells: CCR5-CCR7+-->CCR5+CCR7+-->CCR5+CCR7-. CCR5+CD8+ T cells effectively migrated in response to RANTES, suggesting that CCR5 plays a critical role in the migration of Ag-specific effector and differentiated memory CD8+ T cells to inflammatory tissues and secondary lymphoid tissues. This is in contrast to CCR7, which functions as a homing receptor in migration of naive and memory CD8+ T cells to secondary lymphoid tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
168
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2225-32
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:11859109-Antigens, CD28, pubmed-meshheading:11859109-Antigens, CD45, pubmed-meshheading:11859109-Cell Differentiation, pubmed-meshheading:11859109-Cell Lineage, pubmed-meshheading:11859109-Chemotaxis, Leukocyte, pubmed-meshheading:11859109-Clone Cells, pubmed-meshheading:11859109-Cytotoxicity Tests, Immunologic, pubmed-meshheading:11859109-Epitopes, T-Lymphocyte, pubmed-meshheading:11859109-HIV Antigens, pubmed-meshheading:11859109-HIV-1, pubmed-meshheading:11859109-Humans, pubmed-meshheading:11859109-Immunologic Memory, pubmed-meshheading:11859109-Immunophenotyping, pubmed-meshheading:11859109-Receptors, CCR5, pubmed-meshheading:11859109-Receptors, CCR7, pubmed-meshheading:11859109-Receptors, Chemokine, pubmed-meshheading:11859109-T-Lymphocyte Subsets, pubmed-meshheading:11859109-T-Lymphocytes, Cytotoxic
pubmed:year
2002
pubmed:articleTitle
Functional expression of the chemokine receptor CCR5 on virus epitope-specific memory and effector CD8+ T cells.
pubmed:affiliation
Division of Viral Immunology, Center for AIDS Research, Kumamoto University, 2-2-1 Honjo, Kyokushi, Kumamoto 860-0811, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't