rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2002-3-7
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pubmed:abstractText |
To determine the rate constants of spontaneous and activated TCR cycling, we examined TCR endo- and exocytosis in the human T cell line Jurkat by three different methods. Using a simple kinetic model for TCR cycling and non-linear regression analyses, we found that the spontaneous endocytic rate constant of the TCR was low (approximately 0.012 min(-1)) whereas the spontaneous exocytic rate constant was similar to that of other cycling receptors (approximately 0.055 min(-1)). Following protein kinase C activation (PKC) the endocytic rate constant was increased tenfold (to approximately 0.128 min(-1)) whereas the exocytic rate constant was unaffected. Thus, the TCR becomes a rapidly cycling receptor with kinetics similar to classical cycling receptors subsequent to PKC activation. This results in a reduction of the half-life of cell surface expressed TCR from approximately 58 to 6 min and allows rapid redistribution of the TCR during T cell activation.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Mar
|
pubmed:issn |
0014-2980
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
616-26
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11857335-Amino Acid Motifs,
pubmed-meshheading:11857335-Antibodies, Monoclonal,
pubmed-meshheading:11857335-Antigens, CD3,
pubmed-meshheading:11857335-Down-Regulation,
pubmed-meshheading:11857335-Endocytosis,
pubmed-meshheading:11857335-Enzyme Activation,
pubmed-meshheading:11857335-Exocytosis,
pubmed-meshheading:11857335-Fluorescein-5-isothiocyanate,
pubmed-meshheading:11857335-Fluorescent Dyes,
pubmed-meshheading:11857335-Humans,
pubmed-meshheading:11857335-Jurkat Cells,
pubmed-meshheading:11857335-Kinetics,
pubmed-meshheading:11857335-Phosphorylation,
pubmed-meshheading:11857335-Protein Kinase C,
pubmed-meshheading:11857335-Protein Processing, Post-Translational,
pubmed-meshheading:11857335-Receptors, Antigen, T-Cell,
pubmed-meshheading:11857335-Up-Regulation
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pubmed:year |
2002
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pubmed:articleTitle |
Endo- and exocytic rate constants for spontaneous and protein kinase C-activated T cell receptor cycling.
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pubmed:affiliation |
Institute of Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|