| pubmed-article:11856330 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11856330 | lifeskim:mentions | umls-concept:C1706586 | lld:lifeskim |
| pubmed-article:11856330 | lifeskim:mentions | umls-concept:C0812215 | lld:lifeskim |
| pubmed-article:11856330 | lifeskim:mentions | umls-concept:C2746015 | lld:lifeskim |
| pubmed-article:11856330 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:11856330 | lifeskim:mentions | umls-concept:C1567954 | lld:lifeskim |
| pubmed-article:11856330 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:11856330 | pubmed:dateCreated | 2002-2-21 | lld:pubmed |
| pubmed-article:11856330 | pubmed:abstractText | RNA aptamers with affinity for the Ras-binding domain (RBD) of Raf-1 were isolated from a degenerate pool by in vitro selection. These aptamers efficiently inhibited the Ras interaction with the Raf-1 RBD, and also inhibited Ras-induced Raf-1 activation in a cell-free system. The RNA aptamer with the most potent inhibitory effect specifically inhibited the Ras-Raf-1 interaction and had no affinity for the RBD of the RGL protein, a homolog of the Ral GDP dissociation stimulator. Although the aptamer was capable of binding to the B-Raf RBD, the RNA did not inhibit the interaction between Ras and the B-Raf RBD. Enzymatic and chemical probing experiments indicated that the aptamer was folded into a pseudoknot structure, and some loop regions of the pseudoknot were located at the binding interface for the Raf-1 RBD. | lld:pubmed |
| pubmed-article:11856330 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11856330 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11856330 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11856330 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11856330 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11856330 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11856330 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11856330 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:11856330 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:11856330 | pubmed:author | pubmed-author:KaziroYoshito... | lld:pubmed |
| pubmed-article:11856330 | pubmed:author | pubmed-author:MizutaniShinS | lld:pubmed |
| pubmed-article:11856330 | pubmed:author | pubmed-author:HiraoIchiroI | lld:pubmed |
| pubmed-article:11856330 | pubmed:author | pubmed-author:YokoyamaShige... | lld:pubmed |
| pubmed-article:11856330 | pubmed:author | pubmed-author:KimotoMichiko... | lld:pubmed |
| pubmed-article:11856330 | pubmed:author | pubmed-author:ShirouzuMikak... | lld:pubmed |
| pubmed-article:11856330 | pubmed:author | pubmed-author:KoideHiroshiH | lld:pubmed |
| pubmed-article:11856330 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11856330 | pubmed:volume | 269 | lld:pubmed |
| pubmed-article:11856330 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11856330 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11856330 | pubmed:pagination | 697-704 | lld:pubmed |
| pubmed-article:11856330 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:11856330 | pubmed:meshHeading | pubmed-meshheading:11856330... | lld:pubmed |
| pubmed-article:11856330 | pubmed:meshHeading | pubmed-meshheading:11856330... | lld:pubmed |
| pubmed-article:11856330 | pubmed:meshHeading | pubmed-meshheading:11856330... | lld:pubmed |
| pubmed-article:11856330 | pubmed:meshHeading | pubmed-meshheading:11856330... | lld:pubmed |
| pubmed-article:11856330 | pubmed:meshHeading | pubmed-meshheading:11856330... | lld:pubmed |
| pubmed-article:11856330 | pubmed:meshHeading | pubmed-meshheading:11856330... | lld:pubmed |
| pubmed-article:11856330 | pubmed:meshHeading | pubmed-meshheading:11856330... | lld:pubmed |
| pubmed-article:11856330 | pubmed:meshHeading | pubmed-meshheading:11856330... | lld:pubmed |
| pubmed-article:11856330 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11856330 | pubmed:articleTitle | Anti-(Raf-1) RNA aptamers that inhibit Ras-induced Raf-1 activation. | lld:pubmed |
| pubmed-article:11856330 | pubmed:affiliation | Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan. | lld:pubmed |
| pubmed-article:11856330 | pubmed:publicationType | Journal Article | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11856330 | lld:pubmed |
