Source:http://linkedlifedata.com/resource/pubmed/id/11856330
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2002-2-21
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pubmed:abstractText |
RNA aptamers with affinity for the Ras-binding domain (RBD) of Raf-1 were isolated from a degenerate pool by in vitro selection. These aptamers efficiently inhibited the Ras interaction with the Raf-1 RBD, and also inhibited Ras-induced Raf-1 activation in a cell-free system. The RNA aptamer with the most potent inhibitory effect specifically inhibited the Ras-Raf-1 interaction and had no affinity for the RBD of the RGL protein, a homolog of the Ral GDP dissociation stimulator. Although the aptamer was capable of binding to the B-Raf RBD, the RNA did not inhibit the interaction between Ras and the B-Raf RBD. Enzymatic and chemical probing experiments indicated that the aptamer was folded into a pseudoknot structure, and some loop regions of the pseudoknot were located at the binding interface for the Raf-1 RBD.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-2956
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
269
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
697-704
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11856330-Base Sequence,
pubmed-meshheading:11856330-Cell Line,
pubmed-meshheading:11856330-Humans,
pubmed-meshheading:11856330-Molecular Sequence Data,
pubmed-meshheading:11856330-Nucleic Acid Conformation,
pubmed-meshheading:11856330-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:11856330-RNA,
pubmed-meshheading:11856330-ras Proteins
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pubmed:year |
2002
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pubmed:articleTitle |
Anti-(Raf-1) RNA aptamers that inhibit Ras-induced Raf-1 activation.
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pubmed:affiliation |
Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
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pubmed:publicationType |
Journal Article
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