Source:http://linkedlifedata.com/resource/pubmed/id/11855781
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2002-2-21
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| pubmed:abstractText |
bcl-2 expression is associated with the expression of the multidrug resistance molecule (p-gp) and the resistance of leukaemia cells to the induction of apoptosis. The activity of p-gp is the main mechanism of resistance of leukaemia cells to chemotherapy. This study assessed the induction of apoptosis of acute lymphoblastic leukaemia (ALL) and non-Hodgkin's lymphoma (NHL) blastic cells following in vitro treatment with dexamethasone (DXM), vincristine (VCR), and tumour necrosis factor (TNF) in relation to the expression of bcl-2 and p-gp. Common ALL (cALL; n = 24 patients), common ALL with co-expression of myeloid antigens (cALL + My; n = 9), ALL-T (n = 9), and NHL [n = 6 (T type, n = 2; B type, n = 4)] were included. The expression of bcl-2 and p-gp and apoptosis were assayed by flow cytometry. Spontaneous apoptosis was low (< 5%) in cALL and ALL-T and higher (> 8%) in NHL and cALL + My. A high frequency of bcl-2 expression was noted in cALL and cALL + My. A high frequency of p-gp expression was observed in cALL + My, ALL-T, and NHL. There was a reverse association between bcl-2 expression and spontaneous apoptosis. DXM-induced apoptosis was observed in 52.63%, TNF-induced in 42.85%, VCR-induced in 36.36%, and GM-CSF-induced in 33.3% of leukaemia and lymphoma cases. DXM and GM-CSF-driven apoptosis was reversibly associated with bcl-2-expression (bcl-2-dependent mechanism). VCR and TNF-driven apoptosis was not associated with bcl-2 expression, suggesting a different, bcl-2-independent, mechanism(s) of its induction. The in vitro induction of apoptosis was not associated with expression of p-gp.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/P-Glycoprotein,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Vincristine
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| pubmed:status |
MEDLINE
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| pubmed:issn |
0017-6559
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
31
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
191-207
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11855781-Adolescent,
pubmed-meshheading:11855781-Antineoplastic Agents,
pubmed-meshheading:11855781-Apoptosis,
pubmed-meshheading:11855781-Bone Marrow Cells,
pubmed-meshheading:11855781-Child,
pubmed-meshheading:11855781-Child, Preschool,
pubmed-meshheading:11855781-Dexamethasone,
pubmed-meshheading:11855781-Female,
pubmed-meshheading:11855781-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:11855781-Humans,
pubmed-meshheading:11855781-Lymph Nodes,
pubmed-meshheading:11855781-Lymphoma, Non-Hodgkin,
pubmed-meshheading:11855781-Male,
pubmed-meshheading:11855781-P-Glycoprotein,
pubmed-meshheading:11855781-Precursor Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:11855781-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:11855781-Tumor Necrosis Factor-alpha,
pubmed-meshheading:11855781-Vincristine
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| pubmed:year |
2001
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| pubmed:articleTitle |
Induction of apoptosis and bcl-2 expression in acute lymphoblastic leukaemia and non-Hodgkin's lymphoma in children.
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| pubmed:affiliation |
Department of Clinical Immunology, Polish-American Children's Hospital, Jagiellonian University Medical College, Kraków. mipituch@cyf-kr.edu.pl
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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