Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-2-20
pubmed:abstractText
Proliferative oligodendrocyte progenitor cells (OPs) express large, delayed outward-rectifying K(+) currents (I(K)), whereas nondividing immature and mature oligodendrocytes display much smaller I(K). Here, we show that up-regulation of I(K) occurs in G(1) phase of the cell cycle in purified cultured OPs and is the result of an RNA synthesis-dependent, selective increase of the K(+) channel subunit proteins Kv1.3 and Kv1.5. In oligodendrocyte cells acutely isolated from developing rat brain, a decrease of cyclin D expression is observed as these cells mature along their lineage. This is accompanied by a decrease in Kv1.3 and Kv1.5 subunit expression, suggesting a role for these subunits in the proliferative potential of OPs in situ. I(K) expressed in OPs in subventricular zone and developing white matter in acutely isolated slice preparations were selectively blocked by antagonists of Kv1.3, illustrating the functional presence of this subunit in situ. Interestingly, Kv1.3 block inhibited S-phase entry of both purified OPs in culture and in tissue slice cultures. Thus, we employ both in vitro and in situ experimental approaches to show that (i) RNA-dependent synthesis of Kv1.3 and Kv1.5 subunit proteins occurs in G(1) phase of the OP cell cycle and is responsible for the observed increase in I(K), and (ii) currents through Kv1.3-containing channels play a crucial role in G(1)/S transition of proliferating OPs.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-10377348, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-10414301, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-10528121, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-10594052, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-10601327, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-10607427, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-10825165, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-10884308, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-11160398, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-1326605, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-1453485, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-1691005, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-1724138, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-1791837, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-1941072, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-2560386, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-6300116, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-7612237, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-8187632, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-8204618, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-8352944, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-8439409, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-8551335, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-8999950, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-9092588, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-9298991, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-9308962, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-9334399, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-9654007, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-9655812, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-9777732, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-9792181, http://linkedlifedata.com/resource/pubmed/commentcorrection/11854528-9927607
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin D, http://linkedlifedata.com/resource/pubmed/chemical/Cyclins, http://linkedlifedata.com/resource/pubmed/chemical/KCNA3 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/KCNA5 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Kcna3 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Kcna5 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Kv1.3 Potassium Channel, http://linkedlifedata.com/resource/pubmed/chemical/Kv1.5 Potassium Channel, http://linkedlifedata.com/resource/pubmed/chemical/Lysine, http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor, http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, Voltage-Gated, http://linkedlifedata.com/resource/pubmed/chemical/RNA, http://linkedlifedata.com/resource/pubmed/chemical/biocytin
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
99
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2350-5
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
More...