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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2002-3-7
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pubmed:abstractText |
We are developing methods to image molecular and cellular events in living subjects. In this study, we validate imaging of protein-protein interactions in living mice by using bioluminescent optical imaging. We use the well studied yeast two-hybrid system adapted for mammalian cells and modify it to be inducible. We employ the NF-kappaB promoter to drive expression of two fusion proteins (VP16-MyoD and GAL4-ID). We modulate the NF-kappaB promoter through tumor necrosis factor alpha. Firefly luciferase reporter gene expression is driven by the interaction of MyoD and ID through a transcriptional activation strategy. We demonstrate the ability to detect this induced protein-protein interaction in cell culture and image this induced interaction in living mice by using transiently transfected cells. The current approach will be a valuable and potentially generalizable tool to noninvasively and quantitatively image protein-protein interactions in living subjects. The approaches validated should have important implications for the study of protein-protein interactions in cells maintained in their natural in vivo environment as well as for the in vivo evaluation of new pharmaceuticals targeted to modulate protein-protein interactions.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-10051642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-10473186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-10505102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-10700006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-10933067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-11592831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-11710773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-11734653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-11752410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-1846704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-1847505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-2156629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-2547163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-7937952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-8380166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-8791622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-9237989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-9461201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-9563508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-9624136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-9766640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-9766661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11854471-9829598
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GAL4 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Herpes Simplex Virus Protein Vmw65,
http://linkedlifedata.com/resource/pubmed/chemical/ID1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/ID1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Idb1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Inhibitor of Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/MyoD Protein,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3105-10
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11854471-Animals,
pubmed-meshheading:11854471-Cell Culture Techniques,
pubmed-meshheading:11854471-Cell Line, Transformed,
pubmed-meshheading:11854471-DNA-Binding Proteins,
pubmed-meshheading:11854471-Fungal Proteins,
pubmed-meshheading:11854471-Gene Expression,
pubmed-meshheading:11854471-Genes, Reporter,
pubmed-meshheading:11854471-Herpes Simplex Virus Protein Vmw65,
pubmed-meshheading:11854471-Humans,
pubmed-meshheading:11854471-Inhibitor of Differentiation Protein 1,
pubmed-meshheading:11854471-Luciferases,
pubmed-meshheading:11854471-Mice,
pubmed-meshheading:11854471-MyoD Protein,
pubmed-meshheading:11854471-NF-kappa B,
pubmed-meshheading:11854471-Promoter Regions, Genetic,
pubmed-meshheading:11854471-Proteins,
pubmed-meshheading:11854471-Rats,
pubmed-meshheading:11854471-Recombinant Fusion Proteins,
pubmed-meshheading:11854471-Repressor Proteins,
pubmed-meshheading:11854471-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:11854471-Transcription Factors,
pubmed-meshheading:11854471-Tumor Cells, Cultured,
pubmed-meshheading:11854471-Tumor Necrosis Factor-alpha
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pubmed:year |
2002
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pubmed:articleTitle |
Noninvasive quantitative imaging of protein-protein interactions in living subjects.
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pubmed:affiliation |
The Crump Institute for Molecular Imaging, University of California Los Angeles School of Medicine, Los Angeles, CA 90095, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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