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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
2002-4-29
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pubmed:abstractText |
Recent studies have shown that G-protein-coupled receptors (GPCRs) can assemble as high molecular weight homo- and hetero-oligomeric complexes. This can result in altered receptor-ligand binding, signaling, or intracellular trafficking. We have co-transfected HEK-293 cells with differentially epitope-tagged GPCRs from different subfamilies and determined whether oligomeric complexes were formed by co-immunoprecipitation and immunoblot analysis. This gave the surprising result that the 5HT(1A) receptor was capable of forming hetero-oligomers with all GPCRs tested including the 5HT(1B), 5HT(1D), EDG(1), EDG(3), GPR(26), and GABA(B2) receptors. The testing of other GPCR combinations showed similar results with hetero-oligomer formation occurring for the 5HT(1D) with the 5HT(1B) and EDG(1) receptor. Control studies showed that these complexes were present in co-transfected cells before the time of lysis and that the hetero-oligomers were comprised of GPCRs at discrete stoichiometries. These findings suggest that GPCRs have a natural tendency to form oligomers when co-transfected into cells. Future studies should therefore investigate the presence and physiological role of GPCR hetero-oligomers in cells in which they are endogenously expressed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HTR1B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myc,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Serotonin, 5-HT1B,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Serotonin, 5-HT1D,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin, 5-HT1,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:BachaJamilJ,
pubmed-author:BeerMargaretM,
pubmed-author:BonnertTimT,
pubmed-author:FentonTimT,
pubmed-author:GuestPaul CPC,
pubmed-author:HealdAnneA,
pubmed-author:KerbyJulieJ,
pubmed-author:McAllisterGeorgeG,
pubmed-author:SalimKamranK,
pubmed-author:SkynnerHeather AHA,
pubmed-author:Urien-RodriguezHectorH,
pubmed-author:WattsEmmaE
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15482-5
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:11854302-Cell Line,
pubmed-meshheading:11854302-GTP-Binding Proteins,
pubmed-meshheading:11854302-Humans,
pubmed-meshheading:11854302-Immunoblotting,
pubmed-meshheading:11854302-Kidney,
pubmed-meshheading:11854302-Mutagenesis, Site-Directed,
pubmed-meshheading:11854302-Protein Subunits,
pubmed-meshheading:11854302-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:11854302-Receptor, Serotonin, 5-HT1B,
pubmed-meshheading:11854302-Receptor, Serotonin, 5-HT1D,
pubmed-meshheading:11854302-Receptors, Cell Surface,
pubmed-meshheading:11854302-Receptors, Serotonin,
pubmed-meshheading:11854302-Receptors, Serotonin, 5-HT1,
pubmed-meshheading:11854302-Recombinant Proteins,
pubmed-meshheading:11854302-Restriction Mapping,
pubmed-meshheading:11854302-Transfection
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pubmed:year |
2002
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pubmed:articleTitle |
Oligomerization of G-protein-coupled receptors shown by selective co-immunoprecipitation.
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pubmed:affiliation |
Neuroscience Research Centre, Merck Sharp & Dohme Research Laboratories, Harlow, Essex CM20 2QR, United Kingdom. kamran_salim@merck.com
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pubmed:publicationType |
Journal Article
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