Linked Life Data

11853891

Source:http://linkedlifedata.com/resource/pubmed/id/11853891

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2002-2-20
pubmed:abstractText
A single shock wave generated by a shock tube is able to effectively deliver macromolecules such as fluorescein isothiocyanate-dextran into the cytoplasm of living cells without causing cytotoxicity. We report on the effect of varying the molecular weight of the dextran and the number of shock waves on the efficiency of delivery into a cancer cell line. The fraction of cells permeabilized and the total fluorescence delivered were measured by flow cytometry, and the cellular viability by a tetrazolium assay on adherent cells and these values were compared to cell permeabilization using digitonin. Shock waves can deliver molecules of up to 2000000 molecular weight into the cytoplasm of cells without toxicity and may have applications in gene therapy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
1542
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
186-94
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Shock wave-mediated molecular delivery into cells.
pubmed:affiliation
BAR314B, Wellman Laboratories of Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA. kodama@helix.mgh.harvard.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't