Source:http://linkedlifedata.com/resource/pubmed/id/11853680
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2002-3-6
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pubmed:abstractText |
Two types of Na(+)-independent Mg(2+) efflux exist in erythrocytes: (1) Mg(2+) efflux in sucrose medium and (2) Mg(2+) efflux in high Cl(-) media such as KCl-, LiCl- or choline Cl-medium. The mechanism of Na(+)-independent Mg(2+) efflux in choline Cl medium was investigated in this study. Non-selective transport by the following transport mechanisms has been excluded: K(+),Cl(-)- and Na(+),K(+),Cl(-)-symport, Na(+)/H(+)-, Na(+)/Mg(2+)-, Na(+)/Ca(2+)- and K(+)(Na(+))/H(+) antiport, Ca(2+)-activated K(+) channel and Mg(2+) leak flux. We suggest that, in choline Cl medium, Na(+)-independent Mg(2+) efflux can be performed by non-selective transport via the choline exchanger. This was supported through inhibition of Mg(2+) efflux by hemicholinum-3 (HC-3), dodecyltrimethylammonium bromide (DoTMA) and cinchona alkaloids, which are inhibitors of the choline exchanger. Increasing concentrations of HC-3 inhibited the efflux of choline and efflux of Mg(2+) to the same degree. The K(d) value for inhibition of [(14)C]choline efflux and for inhibition of Mg(2+) efflux by HC-3 were the same within the experimental error. Inhibition of choline efflux and of Mg(2+) efflux in choline medium occurred as follows: quinine>cinchonine>HC-3>DoTMA. Mg(2+) efflux was reduced to the same degree by these inhibitors as was the [(14)C]choline efflux.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Choline,
http://linkedlifedata.com/resource/pubmed/chemical/Cinchona Alkaloids,
http://linkedlifedata.com/resource/pubmed/chemical/Hemicholinium 3,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Modulators,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Quaternary Ammonium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/choline transporter,
http://linkedlifedata.com/resource/pubmed/chemical/dodecyltrimethylammonium
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
1559
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
135-44
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:11853680-Animals,
pubmed-meshheading:11853680-Choline,
pubmed-meshheading:11853680-Cinchona Alkaloids,
pubmed-meshheading:11853680-Down-Regulation,
pubmed-meshheading:11853680-Erythrocytes,
pubmed-meshheading:11853680-Hemicholinium 3,
pubmed-meshheading:11853680-Magnesium,
pubmed-meshheading:11853680-Male,
pubmed-meshheading:11853680-Membrane Transport Modulators,
pubmed-meshheading:11853680-Membrane Transport Proteins,
pubmed-meshheading:11853680-Quaternary Ammonium Compounds,
pubmed-meshheading:11853680-Rats,
pubmed-meshheading:11853680-Rats, Wistar
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pubmed:year |
2002
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pubmed:articleTitle |
Role of the choline exchanger in Na(+)-independent Mg(2+) efflux from rat erythrocytes.
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pubmed:affiliation |
Institut für Klinische Physiologie, Klinikum Benjamin Franklin, Freie Universität Berlin, Germany. hans.ebel@medizin.fu-berlin.de
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pubmed:publicationType |
Journal Article
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