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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2002-2-20
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pubmed:abstractText |
E2 enzymes catalyze attachment of ubiquitin and ubiquitin-like proteins to lysine residues directly or through E3-mediated reactions. The small ubiquitin-like modifier SUMO regulates nuclear transport, stress response, and signal transduction in eukaryotes and is essential for cell-cycle progression in yeast. In contrast to most ubiquitin conjugation, the SUMO E2 enzyme Ubc9 is sufficient for substrate recognition and lysine modification of known SUMO targets. Crystallographic analysis of a complex between mammalian Ubc9 and a C-terminal domain of RanGAP1 at 2.5 A reveals structural determinants for recognition of consensus SUMO modification sequences found within SUMO-conjugated proteins. Structure-based mutagenesis and biochemical analysis of Ubc9 and RanGAP1 reveal distinct motifs required for substrate binding and SUMO modification of p53, IkappaBalpha, and RanGAP1.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
108
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
345-56
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11853669-Amino Acid Sequence,
pubmed-meshheading:11853669-Animals,
pubmed-meshheading:11853669-Binding Sites,
pubmed-meshheading:11853669-GTPase-Activating Proteins,
pubmed-meshheading:11853669-Humans,
pubmed-meshheading:11853669-Ligases,
pubmed-meshheading:11853669-Models, Molecular,
pubmed-meshheading:11853669-Molecular Sequence Data,
pubmed-meshheading:11853669-Mutagenesis, Site-Directed,
pubmed-meshheading:11853669-Protein Conformation,
pubmed-meshheading:11853669-SUMO-1 Protein,
pubmed-meshheading:11853669-Sequence Alignment,
pubmed-meshheading:11853669-Structure-Activity Relationship,
pubmed-meshheading:11853669-Substrate Specificity,
pubmed-meshheading:11853669-Ubiquitin-Conjugating Enzymes
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pubmed:year |
2002
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pubmed:articleTitle |
Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1.
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pubmed:affiliation |
Biochemistry Department, Structural Biology Program, Weill Medical College of Cornell University, New York, NY 10021, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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