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rdf:type |
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lifeskim:mentions |
umls-concept:C0018497,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0030685,
umls-concept:C0035820,
umls-concept:C0039062,
umls-concept:C0205263,
umls-concept:C0391871,
umls-concept:C0456962,
umls-concept:C0596235,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1710082,
umls-concept:C1963578
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pubmed:issue |
Pt 1
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pubmed:dateCreated |
2002-2-18
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pubmed:abstractText |
Inner hair cells of the mammalian cochlea translate acoustic stimuli into 'phase-locked' nerve impulses with frequencies of up to at least 1 kHz. Little is known about the intracellular Ca2+ signal that links transduction to the release of neurotransmitter at the afferent synapse. Here, we use confocal microscopy to provide evidence that Ca2+-induced Ca2+ release (CICR) may contribute to the mechanism. Line scan images (2 ms repetition rate) of neonatal mouse inner hair cells filled with the fluorescent indicator FLUO-3, revealed a transient increase in intracellular Ca2+ concentration ([Ca2+]i) during brief (5-50 ms) depolarizing commands under voltage clamp. The amplitude of the [Ca2+]i transient depended upon the Ca2+ concentration in the bathing medium in the range 0-1.3 mM. [Ca2+]i transients were confined to a region near the plasma membrane at the base of the cell in the vicinity of the afferent synapses. The change in [Ca2+]i appeared uniform throughout the entire basal sub-membrane space and we were unable to observe hotspots of activity. Both the amplitude and the rate of rise of the [Ca2+]i transient was reduced by external ryanodine (20 microM), an agent that blocks Ca2+ release from the endoplasmic reticulum. Intracellular Cs+, commonly used to record at presynaptic sites, produced a similar effect. We conclude that both ryanodine and intracellular Cs+ block CICR in inner hair cells. We discuss the contribution of CICR to the measured [Ca2+]i transient, the implications for synaptic transmission at the afferent synapse and the significance of its sensitivity to intracellular Cs+.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-10379828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-10639174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-10736317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-11020381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-11100146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-11144362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-11301027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-11425887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-1280836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-1313441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-1675264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-1963424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-2412606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-2422165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-2439729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-4962851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-5456208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-6086899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-6606034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-6875955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-7400048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-7869256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-8046450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-8052623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-8071157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-8663998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-8730578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-8845156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-8982170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-8989656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-9003551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-9284300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-9350624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-9683429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-9685158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-9870944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11850498-9925817
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
539
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15-23
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11850498-Animals,
pubmed-meshheading:11850498-Calcium,
pubmed-meshheading:11850498-Calcium Signaling,
pubmed-meshheading:11850498-Cesium,
pubmed-meshheading:11850498-Electric Conductivity,
pubmed-meshheading:11850498-Hair Cells, Auditory, Inner,
pubmed-meshheading:11850498-Mice,
pubmed-meshheading:11850498-Mice, Inbred Strains,
pubmed-meshheading:11850498-Ryanodine,
pubmed-meshheading:11850498-Synapses,
pubmed-meshheading:11850498-Time Factors
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pubmed:year |
2002
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pubmed:articleTitle |
Fast Ca2+ signals at mouse inner hair cell synapse: a role for Ca2+-induced Ca2+ release.
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pubmed:affiliation |
Department of Physiology, University of Bristol, Bristol, BS8 1TD, UK. helen.kennedy@bristol.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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