Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2002-3-6
pubmed:abstractText
Quantitation of hepatitis C virus (HCV) RNA has become an important tool in different clinical settings and is used extensively for pretreatment evaluation of patients infected chronically with HCV. In this study, the performance characteristics of the third generation branched DNA-based signal amplification assay (bDNA 3.0) for HCV RNA quantitation were established. The new assay version showed an analytical specificity of 98%. Mean intra- and between-run imprecisions were 6.8 and 11.2%, respectively. The assay was linear over its entire dynamic range. Quantitation appeared to be unaffected by the genotypic variability of HCV. A comparison of bDNA 3.0 with the second generation bDNA assay calibrated against the international WHO HCV RNA standard, and the PCR-based Cobas Amplicor HCV Monitor 2.0 revealed a fairly good correlation among the assays. Twenty-nine and 11% of the paired quantitative results differed by more than log(10)0.5 (i.e. three-fold). All three assays after calibration against the WHO standard also yielded clinically comparable results with regard to the tailoring of interferon alpha/ribavirin treatment duration in patients infected by HCV genotypes 1, 4, and 5.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0166-0934
pubmed:author
pubmed:issnType
Print
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
159-68
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11849694-Adolescent, pubmed-meshheading:11849694-Adult, pubmed-meshheading:11849694-Aged, pubmed-meshheading:11849694-Antiviral Agents, pubmed-meshheading:11849694-Branched DNA Signal Amplification Assay, pubmed-meshheading:11849694-Child, pubmed-meshheading:11849694-Female, pubmed-meshheading:11849694-Genetic Variation, pubmed-meshheading:11849694-Genotype, pubmed-meshheading:11849694-Hepacivirus, pubmed-meshheading:11849694-Hepatitis C, Chronic, pubmed-meshheading:11849694-Humans, pubmed-meshheading:11849694-Male, pubmed-meshheading:11849694-Middle Aged, pubmed-meshheading:11849694-Polymerase Chain Reaction, pubmed-meshheading:11849694-RNA, Viral, pubmed-meshheading:11849694-Reference Standards, pubmed-meshheading:11849694-Reproducibility of Results, pubmed-meshheading:11849694-Sensitivity and Specificity
pubmed:year
2002
pubmed:articleTitle
Quantitation of hepatitis C virus RNA by third generation branched DNA-based signal amplification assay.
pubmed:affiliation
Institute of Virology, National Reference Centre for Hepatitis C, University of Essen, Hufelandstr. 55, D-45122, Essen, Germany. stefan.ross@uni-essen.de
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Evaluation Studies