Source:http://linkedlifedata.com/resource/pubmed/id/11848616
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2002-2-18
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| pubmed:abstractText |
We have developed a new and simple flow cytometric method to detect damaged red blood cells (RBCs) using anti-Hb in hypotonic solution. We studied a total of 200 patients, including 62 patients with schistocytosis, 8 postsplenectomy patients, and 108 healthy controls. Peripheral blood (2 microl) was stained with phycoerythrin-conjugated (PE) antihemoglobin antibody (anti-Hb) in 0.6% (w/v) NaCl solution, and analyzed by flow cytometry omitting the washing step. The proportion of RBCs stained by anti-Hb was 0.55% (SD +/-0.23%) in normal controls and was significantly higher in patients with schistocytosis (2.95+/-2.95%, p <0.001). Six of 108 blood samples from normal controls and 60 of 62 samples from schistocytosis patients showed > or =1.01% stained RBCs (ie, values > mean+2SD of normal controls). The number of schistocytes counted by microscopic examination correlated with the proportion of RBCs stained by anti-Hb (r = 0.637, p <0.001). The proportions of stained RBCs in blood samples with malaria, spherocytosis, and elliptocytosis were also significantly higher than in normal controls. However, the results in postsplenectomy and iron-deficiency anemia (IDA) patients were not significantly different from the normal controls; the number of schistocytes in postsplenectomy patients was not related to the proportion of RBCs stained by anti-Hb. Based on these findings, flow cytometry of damaged RBCs using anti-Hb in hypotonic solution is a simple, sensitive, and accurate method to detect active hemolysis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
0091-7370
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| pubmed:author |
pubmed-author:HanKyungjaK,
pubmed-author:KangChang SukCS,
pubmed-author:KimMyungshinM,
pubmed-author:KimSo-YoungSY,
pubmed-author:KimYonggooY,
pubmed-author:LeeAhwonA,
pubmed-author:LeeEun JungEJ,
pubmed-author:LeeKyo YoungKY,
pubmed-author:LeeWonbaeW,
pubmed-author:LimJihyangJ,
pubmed-author:PaiSoo HwanSH
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| pubmed:issnType |
Print
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| pubmed:volume |
32
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
37-43
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:11848616-Anemia, Hemolytic,
pubmed-meshheading:11848616-Anemia, Iron-Deficiency,
pubmed-meshheading:11848616-Antibodies,
pubmed-meshheading:11848616-Elliptocytosis, Hereditary,
pubmed-meshheading:11848616-Erythrocytes,
pubmed-meshheading:11848616-Erythrocytes, Abnormal,
pubmed-meshheading:11848616-Flow Cytometry,
pubmed-meshheading:11848616-Hemoglobins,
pubmed-meshheading:11848616-Hemolysis,
pubmed-meshheading:11848616-Humans,
pubmed-meshheading:11848616-Hypotonic Solutions,
pubmed-meshheading:11848616-Malaria,
pubmed-meshheading:11848616-Osmotic Fragility,
pubmed-meshheading:11848616-Postoperative Period,
pubmed-meshheading:11848616-Reference Values,
pubmed-meshheading:11848616-Sensitivity and Specificity,
pubmed-meshheading:11848616-Sodium Chloride,
pubmed-meshheading:11848616-Splenectomy,
pubmed-meshheading:11848616-Staining and Labeling,
pubmed-meshheading:11848616-Time Factors
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| pubmed:year |
2002
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| pubmed:articleTitle |
Rapid, sensitive diagnosis of hemolytic anemia using antihemoglobin antibody in hypotonic solution.
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| pubmed:affiliation |
Department of Clinical Pathology and Pediatrics, Catholic University Medical College, Seoul, Korea.
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| pubmed:publicationType |
Journal Article
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