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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2002-2-18
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pubmed:abstractText |
Intrinsic chemoresistance constitutes a major problem in the therapy of malignant gliomas. In vitro experiments with four astrocytoma/glioblastoma (AC/GBM) cell lines revealed that the chemoresistance of two cell lines, A172 and T98G, to cisplatin and etoposide was due to resistance to drug-induced apoptosis. In contrast, all the AC/GBM cell lines tested were sensitive to treatment with the lipophilic ether lipid erucylphosphocholine, ErPC. ErPC-induced apoptosis was independent of wild-type p53-signaling and triggering of the CD95/CD95 ligand (CD95L) system. Inhibition of protein and RNA synthesis by cycloheximide and actinomycin D did not abrogate ErPC-induced apoptosis. However, expression of members of the bcl-2 protein family was modulated during ErPC treatment. Activation of caspase 3 and mitochondrial alterations were central to ErPC-induced apoptosis. We conclude that ErPC-induced activation of the mitochondrial pathway enables cell death in the chemoresistant AC/GBM cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/BCL2L1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Bcl2l1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Casp3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Cisplatin,
http://linkedlifedata.com/resource/pubmed/chemical/Etoposide,
http://linkedlifedata.com/resource/pubmed/chemical/FASLG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fas Ligand Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphorylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfsf6 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-X Protein,
http://linkedlifedata.com/resource/pubmed/chemical/erucylphosphocholine
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pubmed:status |
MEDLINE
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pubmed:issn |
0250-7005
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3389-96
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11848499-Animals,
pubmed-meshheading:11848499-Antigens, CD95,
pubmed-meshheading:11848499-Antineoplastic Agents,
pubmed-meshheading:11848499-Apoptosis,
pubmed-meshheading:11848499-Astrocytoma,
pubmed-meshheading:11848499-Caspase 3,
pubmed-meshheading:11848499-Caspases,
pubmed-meshheading:11848499-Cisplatin,
pubmed-meshheading:11848499-Down-Regulation,
pubmed-meshheading:11848499-Drug Resistance, Multiple,
pubmed-meshheading:11848499-Drug Resistance, Neoplasm,
pubmed-meshheading:11848499-Enzyme Activation,
pubmed-meshheading:11848499-Etoposide,
pubmed-meshheading:11848499-Fas Ligand Protein,
pubmed-meshheading:11848499-Glioblastoma,
pubmed-meshheading:11848499-Humans,
pubmed-meshheading:11848499-Intracellular Membranes,
pubmed-meshheading:11848499-Membrane Glycoproteins,
pubmed-meshheading:11848499-Membrane Potentials,
pubmed-meshheading:11848499-Mitochondria,
pubmed-meshheading:11848499-Phosphorylcholine,
pubmed-meshheading:11848499-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:11848499-Rats,
pubmed-meshheading:11848499-Signal Transduction,
pubmed-meshheading:11848499-Tumor Cells, Cultured,
pubmed-meshheading:11848499-bcl-X Protein
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pubmed:articleTitle |
Erucylphosphocholine-induced apoptosis in chemoresistant glioblastoma cell lines: involvement of caspase activation and mitochondrial alterations.
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pubmed:affiliation |
Department of Physiology, University of Tübingen, Medical School, Germany. verena.jendrossek@uni-tuebingen.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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