Source:http://linkedlifedata.com/resource/pubmed/id/11848289
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2002-2-18
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pubmed:abstractText |
We isolated eight saponins, a hexacyclic lanosterol tetraglycoside (1), a 27-norlanosterol tetraglycoside (2) and six spirostanol oligoglycosides (3-8), from the plants of the family Liliaceae. In murine leukaemic L1210 cells, saponins 5 and 7 at a concentration of 1 microM showed potent cytotoxic activity and the activities were in the following decreasing order: 5, 7, 1, 3, 2, 8, 4, 6. At a concentration of 10 microM, not only 5 and 7 but also 3 and 8 markedly caused cell death. The flow cytometric analysis indicated that 7 and 8 caused a concentration- and time-dependent apoptosis of L1210 cells (EC50 value = approximately 5 microM). The morphological observation using a light microscope revealed that both 7 and 8 induced shrinkage in cell soma and chromatin condensation, suggesting apoptotic cell death. Moreover, in agarose gel electrophoretic analysis, a typical apoptotic DNA ladder pattern was observed after treatment with both 7 and 8. These results suggest that 7 and 8 caused the death of L1210 cells through the apoptotic process. These compounds may become powerful pharmacological tools for studying the molecular mechanism of apoptosis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-3573
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
257-62
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11848289-Animals,
pubmed-meshheading:11848289-DNA Fragmentation,
pubmed-meshheading:11848289-Leukemia L1210,
pubmed-meshheading:11848289-Liliaceae,
pubmed-meshheading:11848289-Mice,
pubmed-meshheading:11848289-Plant Extracts,
pubmed-meshheading:11848289-Rhizome,
pubmed-meshheading:11848289-Saponins,
pubmed-meshheading:11848289-Tumor Cells, Cultured
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pubmed:year |
2002
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pubmed:articleTitle |
Potent apoptotic effects of saponins from Liliaceae plants in L1210 cells.
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pubmed:affiliation |
Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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