Linked Life Data

11846875

Source:http://linkedlifedata.com/resource/pubmed/id/11846875

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-2-15
pubmed:abstractText
Raffinose family oligosaccharides (RFO) accumulating during seed development are thought to play a role in the desiccation tolerance of seeds. However, the functions of RFO in desiccation tolerance have not been elucidated. Here we examine the functions of RFO in Arabidopsis thaliana plants under drought- and cold-stress conditions, based on the analyses of function and expression of genes involved in RFO biosynthesis. Sugar analysis showed that drought-, high salinity- and cold-treated Arabidopsis plants accumulate a large amount of raffinose and galactinol, but not stachyose. Raffinose and galactinol were not detected in unstressed plants. This suggests that raffinose and galactinol are involved in tolerance to drought, high salinity and cold stresses. Galactinol synthase (GolS) catalyses the first step in the biosynthesis of RFO from UDP-galactose. We identified three stress-responsive GolS genes (AtGolS1, 2 and 3) among seven Arabidopsis GolS genes. AtGolS1 and 2 were induced by drought and high-salinity stresses, but not by cold stress. By contrast, AtGolS3 was induced by cold stress but not by drought or salt stress. All the GST fusion proteins of GST-AtGolS1, 2 and 3 expressed in Escherichia coli had galactinol synthase activities. Overexpression of AtGolS2 in transgenic Arabidopsis caused an increase in endogenous galactinol and raffinose, and showed reduced transpiration from leaves to improve drought tolerance. These results show that stress-inducible galactinol synthase plays a key role in the accumulation of galactinol and raffinose under abiotic stress conditions, and that galactinol and raffinose may function as osmoprotectants in drought-stress tolerance of plants.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0960-7412
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
417-26
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11846875-Acclimatization, pubmed-meshheading:11846875-Amino Acid Sequence, pubmed-meshheading:11846875-Arabidopsis, pubmed-meshheading:11846875-Arabidopsis Proteins, pubmed-meshheading:11846875-Carbohydrate Sequence, pubmed-meshheading:11846875-Cold Temperature, pubmed-meshheading:11846875-Disaccharides, pubmed-meshheading:11846875-Disasters, pubmed-meshheading:11846875-Escherichia coli, pubmed-meshheading:11846875-Galactosyltransferases, pubmed-meshheading:11846875-Gene Expression, pubmed-meshheading:11846875-Molecular Sequence Data, pubmed-meshheading:11846875-Molecular Structure, pubmed-meshheading:11846875-Phylogeny, pubmed-meshheading:11846875-Plant Transpiration, pubmed-meshheading:11846875-Plants, Genetically Modified, pubmed-meshheading:11846875-Raffinose, pubmed-meshheading:11846875-Salts, pubmed-meshheading:11846875-Seeds, pubmed-meshheading:11846875-Sequence Homology, Amino Acid, pubmed-meshheading:11846875-Soil, pubmed-meshheading:11846875-Transcription Factors, pubmed-meshheading:11846875-Water
pubmed:year
2002
pubmed:articleTitle
Important roles of drought- and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana.
pubmed:affiliation
Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't