Linked Life Data

11843817

Source:http://linkedlifedata.com/resource/pubmed/id/11843817

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-2-14
pubmed:abstractText
Chronic lymphocytic leukaemia (CLL) remains an incurable disease. Although modern available treatments are able to induce disease regression, relapse almost inexorably occurs. Therefore, novel therapeutic strategies aimed at reducing the disease relapse rate are very much needed. Among these, the induction of tumour-associated antigen-specific cytotoxic T lymphocytes (CTL), through either DNA vaccines or injection of idiotype pulsed dendritic cells (DCs), has been actively investigated with encouraging preliminary results in B-cell malignancies. As the CLL B lymphocyte characteristically expresses low amounts of surface immunoglobulin (Ig) and T cells from these patients have been reported to display impaired functional activity, there are concerns related to the possibility of generating specific cytotoxic antitumoral T cells in this disease. In addition, no information is presently available regarding the functional ability of CLL-derived DCs. In the present work, freshly purified monocytes from CLL patients and normal donors were induced to differentiate in granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 serum-free medium and compared for their morphological, phenotypic and functional characteristics. Our results demonstrate that: (1) functional DCs can be generated from CLL patients with similar phenotype and function to those observed from normal donors; (2) in contrast to normal control subjects, monocyte-derived DCs from CLL patients spontaneously secrete endogenous IL-10; and (3) interferon (IFN)-gamma in combination with CD40L plays a major role in priming DCs from CLL patients for IL-12 and IL-15 production. Overall, these results indicate that it is possible to derive functionally competent DCs from circulating monocytes in CLL patients.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0007-1048
pubmed:author
pubmed:issnType
Print
pubmed:volume
115
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
831-44
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11843817-Aged, pubmed-meshheading:11843817-Aged, 80 and over, pubmed-meshheading:11843817-CD40 Ligand, pubmed-meshheading:11843817-Cell Differentiation, pubmed-meshheading:11843817-Cells, Cultured, pubmed-meshheading:11843817-Culture Media, Serum-Free, pubmed-meshheading:11843817-Dendritic Cells, pubmed-meshheading:11843817-Female, pubmed-meshheading:11843817-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:11843817-Humans, pubmed-meshheading:11843817-Immunotherapy, Active, pubmed-meshheading:11843817-Interferon-gamma, pubmed-meshheading:11843817-Interleukin-10, pubmed-meshheading:11843817-Interleukin-12, pubmed-meshheading:11843817-Interleukin-15, pubmed-meshheading:11843817-Interleukin-4, pubmed-meshheading:11843817-Leukemia, Lymphocytic, Chronic, B-Cell, pubmed-meshheading:11843817-Monocytes
pubmed:year
2001
pubmed:articleTitle
Functional monocyte-derived dendritic cells can be generated in chronic lymphocytic leukaemia.
pubmed:affiliation
Unité d'Immuno-Hématologie et d'Immunopathologie, Institut Pasteur, 28 rue du Dr Roux, F-75724 Paris Cedex 15, France. vuillier@pasteur.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't