Linked Life Data

11842932

Source:http://linkedlifedata.com/resource/pubmed/id/11842932

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2002-2-14
pubmed:abstractText
The salivary gland is considered to be a reservoir of many growth factors in rodents. In humans, the epidermal growth factor, basic fibroblast growth factor, and insulin and insulin-like growth factor family have also been detected in this gland, but their physiological role remains unclear. In this study, we focused on bFGF, which is a well-known mitogen for various types of cells, and is present in the salivary gland as well as in saliva. The roles of bFGF in the salivary gland were investigated by three different procedures. First, the effects of bFGF on the salivary gland cells were investigated with a monolayer culture of normal submandibular gland cells. The effects of different concentrations of bFGF on the second passage of these cultured cells were examined. In both human and rat cultured submandibular gland cells, bFGF accelerated the cell proliferation at a concentration of 100 ng/mL or higher. Next, an atrophic model of the rat submandibular gland was used to examine the ability of bFGF to accelerate tissue repair. Two weeks after ductal ligation, the ligature was removed, and various amounts of bFGF, isoproterenol, or saline were administered via a retrograde duct instillation. Both isoproterenol and bFGF increased acinar and ductal cell proliferation significantly. To determine the role of bFGF in saliva, we investigated its effect on the healing process of oral mucosal defects. Four-millimeter mucosal defects were made to the depth of the periosteum in the rat palate under anesthesia. bFGF or vehicle alone was applied once only at the time of surgery as a suspension. At days 3, 5, and 7 in the bFGF group, significant increases in the degree of re-epithelialization were found in treated groups. These results indicate that its action as a mitogen stimulus is the major effect of bFGF on salivary gland cells and mucosal epithelium.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0895-9374
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
99-102
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11842932-Animals, pubmed-meshheading:11842932-Atrophy, pubmed-meshheading:11842932-Cell Division, pubmed-meshheading:11842932-Cells, Cultured, pubmed-meshheading:11842932-Disease Models, Animal, pubmed-meshheading:11842932-Epithelium, pubmed-meshheading:11842932-Fibroblast Growth Factor 2, pubmed-meshheading:11842932-Humans, pubmed-meshheading:11842932-Immunohistochemistry, pubmed-meshheading:11842932-Isoproterenol, pubmed-meshheading:11842932-Male, pubmed-meshheading:11842932-Mouth Mucosa, pubmed-meshheading:11842932-Proliferating Cell Nuclear Antigen, pubmed-meshheading:11842932-Rats, pubmed-meshheading:11842932-Rats, Sprague-Dawley, pubmed-meshheading:11842932-Regeneration, pubmed-meshheading:11842932-Salivary Ducts, pubmed-meshheading:11842932-Salivary Gland Diseases, pubmed-meshheading:11842932-Salivary Glands, pubmed-meshheading:11842932-Sodium Chloride, pubmed-meshheading:11842932-Statistics, Nonparametric, pubmed-meshheading:11842932-Submandibular Gland, pubmed-meshheading:11842932-Sympathomimetics, pubmed-meshheading:11842932-Wound Healing
pubmed:year
2000
pubmed:articleTitle
Salivary growth factors in health and disease.
pubmed:affiliation
Department of Oral and Maxillofacial Surgery, Nagoya University, Postgraduate School of Medicine. kagami@hamon.swmed.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't