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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2002-2-13
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pubmed:abstractText |
The transcription factor c-Maf has been suggested to regulate the activity of gamma-crystallin promoters in lens fibre cells. We here show that the transactivation potential of c-Maf and MafB for the rat gammaD-crystallin Maf-responsive element (gammaD MARE) is dependent upon the cellular context and, using chimeric and single domain mutants, that c-Maf is most likely to be the cognate factor for the gammaD MARE in the lens. Transactivation of the gammaD MARE by c-Maf in lens cells was not enhanced by c-Fos or c-Jun and was not blocked by dominant negative c-Fos or c-Jun constructs. c-Maf can activate the gammaD MARE as a homodimer since activation of the gammaD-crystallin promoter in P19 embryonic carcinoma cells required only c-Maf, but none of a number of c-Fos and c-Jun family members tested. Transactivation by c-Maf was inhibited by activation of protein kinase A (PKA) (by signal transduction agonist forskolin) or of protein kinase C (PKC) (by signal transduction agonist tetradecanoyl phorbol acetate). Site-directed mutagenesis showed that this effect is not mediated by phosphorylation of the consensus PKA/PKC site in the extended DNA-binding domain, but likely involves activation of MAP kinase kinase, as inhibition by PD98059 increased transactivation by c-Maf.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-10089876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-10096021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-10097114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-10383433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-10603348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-10702313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-10885750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-11222774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-11416124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-11747370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-1408802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-1628753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-1768434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-2111017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-2320414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-3653512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-650132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-7628452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-8108109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-8264639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-8364223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-8383235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-8575616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-9038383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-9224592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-9392516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-9397686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-9525857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-9571165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-9642290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11842109-9674710
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Avian Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Crystallins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/L-MAF protein, Gallus gallus,
http://linkedlifedata.com/resource/pubmed/chemical/Maf Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Maf protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/MafB Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Mafb protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-maf,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
975-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11842109-Animals,
pubmed-meshheading:11842109-Animals, Newborn,
pubmed-meshheading:11842109-Avian Proteins,
pubmed-meshheading:11842109-CHO Cells,
pubmed-meshheading:11842109-Cell Line,
pubmed-meshheading:11842109-Cells, Cultured,
pubmed-meshheading:11842109-Cricetinae,
pubmed-meshheading:11842109-Crystallins,
pubmed-meshheading:11842109-DNA-Binding Proteins,
pubmed-meshheading:11842109-Genes, Reporter,
pubmed-meshheading:11842109-Growth Substances,
pubmed-meshheading:11842109-Lens, Crystalline,
pubmed-meshheading:11842109-Maf Transcription Factors,
pubmed-meshheading:11842109-MafB Transcription Factor,
pubmed-meshheading:11842109-Oncogene Proteins,
pubmed-meshheading:11842109-Promoter Regions, Genetic,
pubmed-meshheading:11842109-Proto-Oncogene Proteins,
pubmed-meshheading:11842109-Proto-Oncogene Proteins c-maf,
pubmed-meshheading:11842109-Rats,
pubmed-meshheading:11842109-Response Elements,
pubmed-meshheading:11842109-Signal Transduction,
pubmed-meshheading:11842109-Trans-Activators,
pubmed-meshheading:11842109-Transcription Factor AP-1,
pubmed-meshheading:11842109-Transcription Factors,
pubmed-meshheading:11842109-Transcriptional Activation
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pubmed:year |
2002
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pubmed:articleTitle |
c-Maf, the gammaD-crystallin Maf-responsive element and growth factor regulation.
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pubmed:affiliation |
Department of Biochemistry, University of Nijmegen, Nijmegen, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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