Source:http://linkedlifedata.com/resource/pubmed/id/11836610
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2002-2-11
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pubmed:abstractText |
Several investigators have demonstrated that TGF-beta stimulated clone-22 (TSC-22) regulates cell growth and differentiation, and cell death. TSC-22 is a putative transcriptional regulator containing a leucine zipper-like structure and a nuclear export signal. We previously showed the cytoplasmic localization of TSC-22 and the nuclear translocation of TSC-22 concomitant with induction of apoptosis in salivary gland cancer cells. In the present study, we attempted to identify the active domain of TSC-22 protein that regulated the biological functions of TSC-22. We constructed three mammalian expression vectors, which could produce full length TSC-22 only in cytoplasm, the leucine zipper structure of TSC-22 in both cytoplasm and nucleus, and the leucine zipper structure only in nucleus. Then, we transfected a salivary gland cancer cell line, HSG with these expression vectors, and investigated the growth profile of the transfectants. None of the TSC-22 constructs inhibited the monolayer growth and the anchorage-dependent colony formation of HSG cells. However, the leucine zipper structure of TSC-22 markedly inhibited the anchorage-independent colony formation of HSG cells (p<0.001; one way ANOVA). Full length TSC-22 also suppressed the anchorage-independent colony formation of HSG cells, although the effect of full length TSC-22 was much lower than those of the leucine zipper constructs. These observations suggest that the leucine zipper structure in TSC-22 protein is an active domain that negatively regulates the growth of salivary gland cancer cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Localization Signals,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TSC22D1 protein, human
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pubmed:status |
MEDLINE
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pubmed:issn |
1021-335X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
371-4
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pubmed:dateRevised |
2007-5-10
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pubmed:meshHeading |
pubmed-meshheading:11836610-Amino Acid Motifs,
pubmed-meshheading:11836610-Cell Adhesion,
pubmed-meshheading:11836610-Colony-Forming Units Assay,
pubmed-meshheading:11836610-DNA Primers,
pubmed-meshheading:11836610-Green Fluorescent Proteins,
pubmed-meshheading:11836610-Humans,
pubmed-meshheading:11836610-Leucine Zippers,
pubmed-meshheading:11836610-Luminescent Proteins,
pubmed-meshheading:11836610-Nuclear Localization Signals,
pubmed-meshheading:11836610-Plasmids,
pubmed-meshheading:11836610-Recombinant Fusion Proteins,
pubmed-meshheading:11836610-Repressor Proteins,
pubmed-meshheading:11836610-Salivary Gland Neoplasms,
pubmed-meshheading:11836610-Transfection,
pubmed-meshheading:11836610-Tumor Cells, Cultured
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pubmed:articleTitle |
Leucine zipper structure of TSC-22 (TGF-beta stimulated clone-22) markedly inhibits the anchorage-independent growth of salivary gland cancer cells.
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pubmed:affiliation |
Second Department of Oral and Maxillofacial Surgery, Tokushima University School of Dentistry, Tokushima 770-8504, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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