Source:http://linkedlifedata.com/resource/pubmed/id/11835401
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2002-2-8
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| pubmed:abstractText |
Interactions among growth factors, cells, and extracellular matrix regulate proliferation during normal development and in pathologies such as atherosclerosis. SPARC (secreted protein, acidic, and rich in cysteine) is a matrix-associated glycoprotein that modulates the adhesion and proliferation of vascular cells. In this study, we demonstrate that SPARC inhibits human arterial smooth muscle cell proliferation stimulated by platelet-derived growth factor or by adhesion to monomeric type I collagen. Binding studies with SPARC and SPARC peptides indicate specific and saturable interaction with smooth muscle cells that involves the C-terminal Ca2+-binding region of the protein. We also report that SPARC arrests monomeric collagen-supported smooth muscle cell proliferation in the late G1-phase of the cell cycle in the absence of an effect on cell shape or on levels of cyclin-dependent kinase inhibitors. Cyclin-dependent kinase-2 activity, p107 and cyclin A levels, and retinoblastoma protein phosphorylation are markedly reduced in response to the addition of exogenous SPARC and/or peptides derived from specific domains of SPARC. Thus, SPARC, previously characterized as an inhibitor of platelet-derived growth factor binding to its receptor, also antagonizes smooth muscle cell proliferation mediated by monomeric collagen at the level of cyclin-dependent kinase-2 activity.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Osteonectin,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoblastoma Protein
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| pubmed:status |
MEDLINE
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| pubmed:issn |
0730-2312
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2002 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:volume |
84
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
759-71
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:11835401-Amino Acid Sequence,
pubmed-meshheading:11835401-Animals,
pubmed-meshheading:11835401-Aorta,
pubmed-meshheading:11835401-Cell Cycle,
pubmed-meshheading:11835401-Cell Division,
pubmed-meshheading:11835401-Cell Size,
pubmed-meshheading:11835401-Collagen Type I,
pubmed-meshheading:11835401-Cyclin-Dependent Kinases,
pubmed-meshheading:11835401-Drug Interactions,
pubmed-meshheading:11835401-Enzyme Inhibitors,
pubmed-meshheading:11835401-Extracellular Matrix,
pubmed-meshheading:11835401-G1 Phase,
pubmed-meshheading:11835401-Humans,
pubmed-meshheading:11835401-Mice,
pubmed-meshheading:11835401-Molecular Sequence Data,
pubmed-meshheading:11835401-Muscle, Smooth, Vascular,
pubmed-meshheading:11835401-Osteonectin,
pubmed-meshheading:11835401-Peptides,
pubmed-meshheading:11835401-Phosphorylation,
pubmed-meshheading:11835401-Platelet-Derived Growth Factor,
pubmed-meshheading:11835401-Retinoblastoma Protein
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| pubmed:year |
2002
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| pubmed:articleTitle |
Inhibition of PDGF-stimulated and matrix-mediated proliferation of human vascular smooth muscle cells by SPARC is independent of changes in cell shape or cyclin-dependent kinase inhibitors.
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| pubmed:affiliation |
Department of Vascular Biology, The Hope Heart Institute, Seattle, Washington 98104, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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