Source:http://linkedlifedata.com/resource/pubmed/id/11830574
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2002-2-6
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| pubmed:abstractText |
The KH domain protein MEX-3 is central to the temporal and spatial control of PAL-1 expression in the C. elegans early embryo. PAL-1 is a Caudal-like homeodomain protein that is required to specify the fate of posterior blastomeres. While pal-1 mRNA is present throughout the oocyte and early embryo, PAL-1 protein is expressed only in posterior blastomeres, starting at the four-cell stage. To better understand how PAL-1 expression is regulated temporally and spatially, we have identified MEX-3 interacting proteins (MIPs) and characterized in detail two that are required for the patterning of PAL-1 expression. RNA interference of MEX-6, a CCCH zinc-finger protein, or SPN-4, an RNA recognition motif protein, causes PAL-1 to be expressed in all four blastomeres starting at the four-cell stage. Genetic analysis of the interactions between these mip genes and the par genes, which provide polarity information in the early embryo, defines convergent genetic pathways that regulate MEX-3 stability and activity to control the spatial pattern of PAL-1 expression. These experiments suggest that par-1 and par-4 affect distinct processes. par-1 is required for many aspects of embryonic polarity, including the restriction of MEX-3 and MEX-6 activity to the anterior blastomeres. We find that PAL-1 is not expressed in par-1 mutants, because MEX-3 and MEX-6 remain active in the posterior blastomeres. The role of par-4 is less well understood. Our analysis suggests that par-4 is required to inactivate MEX-3 at the four-cell stage. Thus, PAL-1 is not expressed in par-4 mutants because MEX-3 remains active in all blastomeres. We propose that MEX-6 and SPN-4 act with MEX-3 to translate the temporal and spatial information provided by the early acting par genes into the asymmetric expression of the cell fate determinant PAL-1.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Combinations,
http://linkedlifedata.com/resource/pubmed/chemical/Helminth Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MEX-3 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/MEX-5 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/MEX-6 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/Oils,
http://linkedlifedata.com/resource/pubmed/chemical/P & S Liquid,
http://linkedlifedata.com/resource/pubmed/chemical/PAR-1 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/PAR-3 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/Phenols,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SPN-4 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/pal-1 protein, C elegans
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0950-1991
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
129
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
747-59
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| pubmed:dateRevised |
2010-7-12
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| pubmed:meshHeading |
pubmed-meshheading:11830574-Animals,
pubmed-meshheading:11830574-Caenorhabditis elegans,
pubmed-meshheading:11830574-Caenorhabditis elegans Proteins,
pubmed-meshheading:11830574-Cell Cycle Proteins,
pubmed-meshheading:11830574-Cell Polarity,
pubmed-meshheading:11830574-Drug Combinations,
pubmed-meshheading:11830574-Gene Expression Regulation, Developmental,
pubmed-meshheading:11830574-Genes, Helminth,
pubmed-meshheading:11830574-Helminth Proteins,
pubmed-meshheading:11830574-Homeodomain Proteins,
pubmed-meshheading:11830574-Oils,
pubmed-meshheading:11830574-Phenols,
pubmed-meshheading:11830574-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11830574-RNA-Binding Proteins,
pubmed-meshheading:11830574-Trans-Activators
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| pubmed:year |
2002
|
| pubmed:articleTitle |
MEX-3 interacting proteins link cell polarity to asymmetric gene expression in Caenorhabditis elegans.
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| pubmed:affiliation |
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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