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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-2-6
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pubmed:abstractText |
While most subtypes of glutamate receptors have been studied extensively, less is known about the delta-glutamate receptors, delta1 and delta2. Although neither forms functional channels when expressed in heterologous cells, genetic analyses have demonstrated the physiological significance of delta2. We used the cytosolic C-terminus of the delta2 glutamate receptor subunit in a yeast two-hybrid screen of a rat brain cDNA library to identify delta-glutamate receptor binding proteins. We isolated rat EMAP, the rat homolog of a microtubule-associated protein initially isolated and characterized in echinoderms. Rat EMAP contains 10 WD-repeats, which are domains important for mediating protein-protein interactions in a wide variety of proteins. Rat EMAP binds to delta-glutamate receptor subunits within a 50-amino-acid segment of the delta C-terminus. It is widely expressed in both brain and peripheral tissues, including high expression in brainstem and enrichment in the postsynaptic density.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
©2002 Elsevier Science (USA).
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
291
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
85-90
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pubmed:dateRevised |
2008-7-10
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pubmed:meshHeading |
pubmed-meshheading:11829466-Animals,
pubmed-meshheading:11829466-Brain Stem,
pubmed-meshheading:11829466-Cell Line,
pubmed-meshheading:11829466-Cloning, Molecular,
pubmed-meshheading:11829466-In Situ Hybridization,
pubmed-meshheading:11829466-Microtubule-Associated Proteins,
pubmed-meshheading:11829466-Molecular Sequence Data,
pubmed-meshheading:11829466-Nerve Tissue Proteins,
pubmed-meshheading:11829466-Organ Specificity,
pubmed-meshheading:11829466-Protein Binding,
pubmed-meshheading:11829466-Protein Subunits,
pubmed-meshheading:11829466-Rats,
pubmed-meshheading:11829466-Receptors, Glutamate,
pubmed-meshheading:11829466-Repetitive Sequences, Amino Acid,
pubmed-meshheading:11829466-Sequence Homology, Amino Acid,
pubmed-meshheading:11829466-Synapses,
pubmed-meshheading:11829466-Two-Hybrid System Techniques
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pubmed:year |
2002
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pubmed:articleTitle |
Identification of rat EMAP, a delta-glutamate receptor binding protein.
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pubmed:affiliation |
Laboratory of Neurochemistry, National Institutes of Health, Bethesda, Maryland 20892, USA.
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pubmed:publicationType |
Journal Article
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