Source:http://linkedlifedata.com/resource/pubmed/id/11827754
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2002-2-5
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| pubmed:abstractText |
Extracellular superoxide dismutase (EC-SOD) is neuroprotective, but its role in cerebral ischemia remains to be determined. We herein describe the topographical localization and quantitative changes in EC-SOD and its mRNA expression following cerebral ischemia in mice. Mice were subjected to transient forebrain ischemia and varied intervals of reperfusion. The measurements of EC-SOD using ELISA showed increased brain EC-SOD after 24 h of reperfusion and an increase in EC-SOD brain/serum ratio after 3 h. The immunohistochemical examination in normal mice showed strong EC-SOD immunoreactivity in the choroid plexus, pia mater, and ventral tuberal area of the hypothalamus. EC-SOD immunoreactivity in cortical and striatal capillary wall was conspicuous after 3 h. EC-SOD immunoreactivity was also noted in cortical neurons after 24 h. Northern blot analysis showed an increased EC-SOD mRNA expression in the brain after 24 h. An in situ hybridization study in normal mice demonstrated the mRNA expression of EC-SOD in choroid plexus and neurons through the brain especially in the cortex or ventral tuberal area of the hypothalamus, but demonstrated no mRNA expression of EC-SOD in the capillary wall. These findings suggest that EC-SOD accumulates on endothelial cells in response to this injury by an unknown mechanism, while cortical neurons produce EC-SOD themselves after cerebral ischemia with reperfusion.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0891-5849
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
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| pubmed:volume |
32
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
289-98
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:11827754-Animals,
pubmed-meshheading:11827754-Brain,
pubmed-meshheading:11827754-Brain Ischemia,
pubmed-meshheading:11827754-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11827754-Extracellular Space,
pubmed-meshheading:11827754-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:11827754-Immunohistochemistry,
pubmed-meshheading:11827754-In Situ Hybridization,
pubmed-meshheading:11827754-Male,
pubmed-meshheading:11827754-Mice,
pubmed-meshheading:11827754-Mice, Inbred C57BL,
pubmed-meshheading:11827754-Protein Biosynthesis,
pubmed-meshheading:11827754-RNA, Messenger,
pubmed-meshheading:11827754-Superoxide Dismutase,
pubmed-meshheading:11827754-Transcription, Genetic
|
| pubmed:year |
2002
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| pubmed:articleTitle |
Post-ischemic transcriptional and translational responses of EC-SOD in mouse brain and serum.
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| pubmed:affiliation |
Department of Neurosurgery, National Defense Medical College, Tokorozawa, Japan. grd1309@gr.ndmc.ac.jp
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| pubmed:publicationType |
Journal Article
|